医薬使用のためのリパーゼ変異体
任意にはプロテアーゼ及び/又はアミラーゼと組合しての、配列番号２のアミノ酸1-269を含んで成るサーモミセス・ラヌギノサス（Thermomyces lanuginosus）（ヒューミコラ・ラヌギノサ（Humicola lanuginose））リパーゼに関連するリパーゼの医薬使用に関する。医学的適応の例は、消化異常、膵臓の外分泌不十分（PEI）、膵炎、嚢胞性線維症、I型及びII型糖尿病の処理である。本発明のリパーゼは、例えばインビトロでの改良された消化性能、中性範囲のpHでの改良された活性、低いpHでの改良された安定性、プロテアーゼ分解に対する安定性、及び/又はペプシン及び胆汁塩の存在下での安定性を有する。本発明はまた、リパーゼのインビトロでの消化性能を決定する方法、及びT. ラヌギノサスのリパーゼの一定の新規変異体にも関する。
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【特許請求の範囲】
【請求項１】
（ａ）配列番号２のアミノ酸１〜269の配列に対して少なくとも50％の同一性を有し；
（ｂ）リパーゼ活性を有し；そして
（ｃ）配列番号2のアミノ酸１〜269の配列に比較して、置換N33Q、T231R及びN233R、並びに下記：
E1*,D,N；Q4H,P,R；D5E；N8L,Q；Q9H；F10L; N11C,D,H,L,P,Q,R,S; G23E; N26A,H,I；
D27I,N,Q,R,S,V; P29T; A30T,V; T37K,M; G38A,D,F,H,I,K,L,M,N,P,Q1S,T,W,Y; N39H,S; E43K;K46M; A49T; L52I,R; E56K,Q,R,S; D57G,N; V60E,S; G61R; V63R; A68V; L69I; N71I,S;N73Q,Y; I76T; R84E; I86F,L; E87A,H,K,R; I90L,V; G91A,C,E,F,K,L,M,N,S,T,V,W,Y; L93*,F;N94*K,Q,R,S;F95*;D96*,E,G,N,R,S,W,Y;L97M,Q;K98I,T;E99D;N101Q;D102E,G,Y;
R108M;G109A;D111A,E,N,S;G112A;T114I;S115L;W117C,D,E,F,G,H,I,K,L,P,S,T,V,Y;D122E,N;Q126L;V128A;D130H;H135D;P136H;Y138F;V141E,L;A150V;V154F,I,L;A155V;G156R;G161A,E;N162G,S,T;G163A,C,D,E,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y;D167E;V168M;V176A,D,F,G,H,I,K,M,N,Q,T,W; G177A; R179T; L185M; G190C,D; N200Q,S; R205I;L206F; E210D,R,V,Y; S216P; E219D; G225P; T226N; L227F,G; P229R; E239D; G240L; D242E; T244S; G246A;Q249R;N251Q,S;D254A,G,I,K,L,M,N,R,Q,S,Y;I255A,F;P256A,F,G,H,I,L,M,N,Q,S,T,V,W,Y; 及び L269F,Hから選択された少なくとも１つの追加の置換を含んで成る、薬剤として使用するためのリパーゼ。
【請求項２】
次の置換：N33Q+T231R+N233Rを有する、配列番号２の配列を有する対照リパーゼに比較して、インビトロで改良された消化性能を有する請求項１記載のリパーゼ。
【請求項３】
ａ）対照リパーゼを選択し；
ｂ）それぞれ、100部/体積の食餌と、20部/体積のペプシン及び30部/体積のリパーゼ又は対照リパーゼとを混合し；
ｃ）i)０又はii)10部/体積の緩衝液（0.8MのMES、0.8Mの酢酸ナトリウム、0.8Mのイミダゾール、pH7.0）を添加し、ここで段階i)はpH3の胃段階として言及され得、そして段階ii)はpH5の胃段階として言及され得；
ｄ）振盪下で37℃で１時間インキュベートし；
ｅ）20部/体積の胆汁塩、及びi)25又はii)15部/体積の緩衝液（0.8MのMES、0.8Mの酢酸ナトリウム、0.8Mのイミダゾール、pH7.0）を添加し、ここで段階i)はpH3の胃段階として言及され得、そして段階ii)はpH5の胃段階として言及され得；
ｆ）振盪下で37℃で２時間インキュベートし；
ｇ）1Mのリン酸中、50部/体積の10％Triton-X100を添加し；
ｈ）遊離脂肪酸の体積を決定し；
i)下記等式：
FFA＝FFAmax*[E]/（[E]＋K）
[式中、FFAは開放される遊離脂肪酸の量であり、FFAmaxは、リパーゼ食餌から遊離することができる遊離脂肪酸の最大量であり、「E」はリパーゼ濃度であり、そしてKはFFAmaxの半分を遊離するリパーゼ濃度である]に、用量応答曲線を適合し；
ｊ）次の通りに改良因子（IF）：
IF＝K（対照）/K(リパーゼ)
[ここでK（対照）は、FFAmaxの半分を遊離する対照リパーゼの濃度であり、そしてK（リパーゼ）は、FFAmaxの半分を遊離するリパーゼ濃度である]を計算する段階を含んで成る、対照リパーゼに比較して、リパーゼのインビトロ消化性能を決定するための方法。
【請求項４】
ａ）i)対照リパーゼを選択し；
ii)それぞれ、５部/体積のリパーゼ又は対照リパーゼと、５部/体積の
ａ）0.001％のTriton−X100及び10mMのNaClを含む希釈剤、又は
ｂ）150μg/mlのペプシン、4mMのCaCl2、0.01％のTriton-X100及び50mMのシトレート、pH3.0を含むペプシン処理溶液（ここでa）は未処理のサンプルとして言及され、そしてｂ）はペプシン処理されたサンプルとして言及される）とを混合し；
iii)段階ii)のサンプルを、20℃で３時間インキュベートし；
iv)段階iii)の個々のサンプルに、1mMのPNP−パルミテート、1.2％のTriton-X100、4mMのCaCl2、100mMのTRIS, pH8.0を含む、55部/体積の基質、及び
ａ）5部/体積のペプシン処理溶液、又は
ｂ）5部/体積の希釈剤（ここでａ）は未処理のサンプルを言及し、そしてｂ）はペプシン処理されたサンプルを言及する）を添加し；
v）iv)サンプルのOD405を一定間隔で読み取ることにより基質の分解を追跡し；
vi)直線範囲に落ちるv）からのデータを集め、そしてそれぞれ、ペプシン処理されたサンプル及び未処理のサンプルについて、mOD（ミリOD）/時でのリパーゼ活性を計算し；
vii)ペプシン処理されたサンプルのリパーゼ活性を、未処理のサンプルのリパーゼ活性により、それらが段階vi)に起因する場合、割算し、そしてその結果に100を掛算することにより％残留リパーゼ活性（％RA）を計算し；そして所望には、
viii)リパーゼの％RAと、対照リパーゼの％RAとを比較することにより、ペプシンの存在下でpH3での安定性を決定し、そして/又は
ｂ）i)対照サンプルを選択し；
ii)それぞれ、10部/体積のリパーゼ又は対照リパーゼと、23部/体積のａ）水、又はｂ）20mMの胆汁塩とを混合し、ここでａ）は未処理のサンプルとして言及され、そしてｂ）は胆汁塩サンプルとして言及され；
iii)ii)の個々のサンプルに、25mMのスクシネート中、1mMのPNPオレエート、2mMのCaCl2、1.2％のTriton−X100、pH5.0を含む、200部/体積の基質を転化し；
iv)段階iii)の直後、個々のサンプルから、60部/体積の前記得られる混合物を除き、そしてその15部/体積を、４つの別々の区画中に４度、移し；
v）１，２，３及び４時間後、60部/体積の100mMのTRIS（pH8.0）を、iv)の４つの区画のそれぞれの区画に添加し、すぐにOD405を読み取り、そして１，２，３及び４時間の読み取りの線状範囲に基づいて、mOD/時での活性を計算し；
vi)それぞれ、リパーゼ及び対照リパーゼの胆汁塩安定性比率に達するよう、リパーゼ及び対照リパーゼについて、胆汁塩サンプルの段階v)で得られる活性を、段階v）でまた得られるような未処理のサンプルの活性により割算し；そして
vii)リパーゼの胆汁塩安定性比率を、対照リパーゼの胆汁塩を安定性比率により割算する（得られる比率はリパーゼの改良因子として定義され得る）ことにより、胆汁塩の存在下でpH5での活性を定義する段階を含んで成る、対照リパーゼに比較して、リパーゼのインビトロでの消化性能を決定するための方法。
【請求項５】
前記インビトロでの消化性能が請求項３又は４のいずれか１項記載の方法を用いて決定される請求項２記載のリパーゼ。
【請求項６】
下記：
D27R+N33Q+G91A+D96E+L97Q+D111A+T231R+N233R+P256T;
N33Q+E210D+T231R+N233R;
N33Q+D111A+T231R+N233R;
N33Q+G91T+T231R+N233R;
N33Q+E219D+T231R+N233R;
N33Q+W117L+T231R+N233R;
D27Q+N33Q+T231R+N233R;
N33Q+G91T+T231R+N233R;
D27S+N33Q+G91A+D96E+L97Q+D111A+S216P+T231R+N233R+P256T;
D27R+N33Q+G91N+N94R+D111A+T231R+N233R+P256T;
D27R+N33Q+G91T+N94S+D111A+S216P+L227G+T231R+N233R+P256T;
Q4R+N33Q+T231R+N233R;
N33Q+T231R+N233R+Q249R;
N33Q+D96W+T231R+N233R;
D27V+N33Q+V60S+D96W+T231R+N233R+Q249R;
D27V+N33Q+V60S+T231R+N233R+Q249R;
Q9H+N33Q+D102E+T231R+N233R;
N33Q+D111E+T231R+N233R;
N33Q+D122E+T231R+N233R;
D27R+N33Q+G91N+N94R+D111A+S216P+L227G+T231R+N233R+P256T;
N33Q+D167E+T231R+N233R;
N33Q+G91N+T231R+N233R;
N33Q+T231R+N233R+P256T;
D27R+N33Q+G91A+L93*+N94*+F95*+D96*+D111A+T231R+N233R+P256T;
N11R+N33Q+T231R+N233R;
N33Q+N39H+T231R+N233R;
N33Q+P229R+T231R+N233R;
D27R+N33Q+G91N+N94R+D111A+G163K+S216P+L227G+T231R+N233R+P256T;
N33Q+G91T+G163K+T231R+N233R;
D27R+N33Q+G91A+D96E+L97Q+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+G91A+D96E+L97Q+D111A+S216P+T231R+N233R+P256T;
N33Q+E87A+T231R+N233R;
N33Q+E56Q+T231R+N233R;
N33Q+E210V+T231R+N233R;
N33Q+E56K+T231R+N233R;
N33Q+T231R+N233R+D254G;
N33Q+D96S+T231R+N233R;
N33Q+D122N+T231R+N233R;
N26A+N33Q+T231R+N233R;
N33Q+N162T+T231R+N233R;
N33Q+A150V+N162G+T231R+N233R;
N33Q+I90L+G163L+T231R+N233R;
N33Q+T231R+N233R+G240L;
D27R+N33Q+G91A+D96E+D111A+T231R+N233R+D254G+P256T;
D27R+N33Q+G91A+N94S+D111A+T231R+N233R+P256T;
N33Q+N200S+T231R+N233R;
N33Q+N39S+T231R+N233R;
N33Q+E210R+T231R+N233R;
N33Q+N39H+T231R+N233R+D254R;
N33Q+T231R+N233R+D254R;
N33Q+N94R+T231R+N233R;
N33Q+D96R+T231R+N233R;
D27N+N33Q+T231R+N233R;
D27N+N33Q+E56R+T231R+N233R;
N33Q+L227F+T231R+N233R;
N33Q+N73Y+G225P+T231R+N233R;
N33Q+G225P+T231R+N233R;
N33Q+T231R+N233R+D254S;
N33Q+D96G+T231R+N233R;
N33Q+D96N+T231R+N233R+D254S;
N33Q+T231R+N233R+D254G;
N33Q+D130H+T231R+N233R;
N33Q+E87A+T231R+N233R;
N33Q+T231R+N233R+E239D;
N33Q+D111A+T231R+N233R+D254G;
N33Q+E210V+T231R+N233R+D254S;
N11R+N33Q+E210V+T231R+N233R+D254S;
N33Q+G91T+G163K+T231R+N233R+D254G;
N33Q+G91T+G163K+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+T231R+N233R+D254S;
Q4R+D27R+N33Q+G91T+N94S+D111A+S216P+L227G+T231R+N233R+P256T;
N33Q+G91T+N94S+D111A+V176I+T231R+N233R;
Q4R+D27R+N33Q+G91T+N94S+D111A+E210D+S216P+L227G+T231R+N233R+P256T;
Q4R+D27Q+N33Q+G91T+N94S+D111A+S216P+L227G+T231R+N233R+P256T;
N33Q+G91T+N94S+D111A+T231R+N233R+P256T;
N33Q+G177A+T231R+N233R;
N33Q+T231R+N233R+G246A;
D27N+N33Q+G91T+G163K+T231R+N233R+D254S;
D27Q+N33Q+G91T+G163K+E219D+T231R+N233R;
N33Q+G91T+E219D+T231R+N233R;
K98I+T231R+N233R+N251S;
N33Q+G163R+T231R+N233R;
N33Q+G163N+T231R+N233R;
N33Q+G163C+T231R+N233R;
N33Q+G163Q+T231R+N233R;
N33Q+G163E+T231R+N233R;
N33Q+G163H+T231R+N233R;
N33Q+G163I+T231R+N233R;
N33Q+G163P+T231R+N233R;
N33Q+G163D+T231R+N233R;
N33Q+G91K+T231R+N233R;
N33Q+G91M+T231R+N233R;
N33Q+G91F+T231R+N233R;
N33Q+G91S+T231R+N233R;
N33Q+G91W+T231R+N233R;
N33Q+G91Y+T231R+N233R;
N33Q+G163T+T231R+N233R;
N33Q+G163W+T231R+N233R;
N33Q+G163Y+T231R+N233R;
N33Q+G163V+T231R+N233R;
N33Q+G91C+T231R+N233R;
N33Q+G91Y+Q126L+T231R+N233R;
N33Q+G91M+G161E+T231R+N233R;
N33Q+V128A+T231R+N233R;
N33Q+G163V+L185M+T231R+N233R;
N33Q+G38A+T231R+N233R;
N33Q+G163A+T231R+N233R;
N33Q+G91T+N94S+D111A+T231R+N233R;
N33Q+G38A+G163A+T231R+N233R;
N33Q+G163M+T231R+N233R;
N33Q+G91V+T231R+N233R;
N33Q+D111A+T231R+N233R+Q249R;
N33Q+D111A+T231R+N233R+D254S;
D27R+N33Q+G91A+D96E+L97Q+D111A+T231R+N233R+D254S+P256T;
D27R+N33Q+G91A+D96E+L97Q+D111A+T231R+N233R+D254G+P256T;
N33Q+G91T+N94R+T231R+N233R+D254S;
N33Q+G91T+N94R+D111A+W117L+T231R+N233R;
N33Q+W117L+T231R+N233R+D254S;
N33Q+T231R+N233R+P256T;
N33Q+T231R+N233R+D242E;
N33Q+E87R+T231R+N233R;
N33Q+E56R+T231R+N233R;
N33Q+N162G+T231R+N233R;
N33Q+G91L+T231R+N233R;
N33Q+E87H+T231R+N233R;
N33Q+D96N+T231R+N233R+Q249R;
N33Q+G91T+N94R+T231R+N233R+D254S;
N33Q+L227F+T231R+N233R+D254S;
D27R+N33Q+G91T+D96E+L97Q+D111A+T231R+N233R+D254S+P256T;
N33Q+G163A+T231R+N233R;
D27R+N33Q+G91T+D96E+D111A+T231R+N233R+D254S+P256T;
N33Q+G91T+N94R+T231R+N233R;
N33Q+T231R+N233R+D254A;
N33Q+T231R+N233R+D254N;
N33Q+T231R+N233R+D254Q;
N33Q+T231R+N233R+D254I;
N33Q+T231R+N233R+D254L;
N33Q+T231R+N233R+D254K;
N33Q+T231R+N233R+D254M;
N33Q+S216P+L227G+T231R+N233R+Q249R;
D27V+N33Q+V60S+G91T+D96W+T231R+N233R+Q249R;
N33Q+D96N+L227G+T231R+N233R+Q249R;
D27R+N33Q+L227G+T231R+N233R;
D27R+N33Q+L227G+T231R+N233R+Q249R;
N33Q+E219D+L227G+T231R+N233R+Q249R;
D27Q+N33Q+L227G+T231R+N233R+Q249R;
N33Q+W117L+L227G+T231R+N233R+Q249R;
D5E+N33Q+W117L+L227G+T231R+N233R+Q249R;
D27Q+N33Q+E219D+L227G+T231R+N233R+Q249R;
N33Q+D96E+E219D+L227G+T231R+N233R+Q249R;
D27R+N33Q+E56K+G91N+N94R+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+E56Q+D57N+G91N+N94R+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+S216P+L227G+T231R+N233R+D254S+P256T;
D27R+N33Q+E56S+G91N+N94R+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+G91N+N94R+D111A+S216P+L227G+T231R+N233R+D254S+P256T;
D27R+N33Q+G91N+N94R+D111A+S216P+L227G+T231R+N233R+D254S+P256T;
D27R+N33Q+G91N+N94R+D111S+A155V+S216P+L227G+T231R+N233R+D254S+P256T;
D27R+N33Q+G91N+N94R+D111S+S216P+L227G+T231R+N233R+D254S+P256T;
N33Q+D111A+T231R+N233R+D254S;
N33Q+D111A+W117L+T231R+N233R+D254S;
N33Q+T231R+N233R+P256A;
N33Q+T231R+N233R+P256N;
N33Q+T231R+N233R+P256G;
N33Q+T231R+N233R+P256H;
N33Q+T231R+N233R+P256L;
N33Q+T231R+N233R+P256M;
N33Q+T231R+N233R+P256S;
N33Q+T231R+N233R+P256W;
N33Q+T231R+N233R+P256Y;
N33Q+T231R+N233R+P256F;
N33Q+T231R+N233R+P256V;
N33Q+G91M+G163W+T231R+N233R;
N33Q+G91M+G163T+T231R+N233R;
N33Q+G91M+G163D+T231R+N233R;
N33Q+G91K+G163W+T231R+N233R;
N33Q+G91T+G163W+T231R+N233R;
N33Q+V176N+T231R+N233R;
N33Q+V176D+T231R+N233R;
N33Q+W117F+T231R+N233R;
N33Q+G91T+N94S+D111A+V176I+T231R+N233R+D254S;
N33Q+V176I+T231R+N233R;
N33Q+D111N+T231R+N233R;
N33Q+D111N+G225P+T231R+N233R;
N33Q+D111N+S216P+T231R+N233R;
D27R+N33Q+G91T+N94R+D111A+S216P+L227G+T231R+N233R;
N33Q+G91M+G163P+T231R+N233R;
N33Q+G91T+G163A+T231R+N233R;
N33Q+W117D+T231R+N233R;
N33Q+W117H+T231R+N233R;
N33Q+W117C+T231R+N233R;
N33Q+W117K+T231R+N233R;
N33Q+W117V+T231R+N233R;
N11S+N33Q+T231R+N233R;
N33Q+W117E+V176K+T231R+N233R;
N33Q+W117G+T231R+N233R;
N33Q+W117P+T231R+N233R;
N33Q+W117S+T231R+N233R;
N33Q+ W117T+T231R+N233R;
N33Q+W117I+T231R+N233R;
D27R+N33Q+ L227G +T231R+N233R+Q249R+D254S;
N33Q+S115L+T231R+N233R;
N33Q+G38A+G91T+G163K+T231R+N233R+D254S;
N33Q+V176M+T231R+N233R;
N33Q+V176H+T231R+N233R;
N33Q+V176A+T231R+N233R;
D27V+N33Q+L227F+T231R+N233R+Q249R;
N33Q+W117Y+T231R+N233R;
N33Q+W117Y+V176D+T231R+N233R;
D27V+N33Q+G91A+N94R+D111A+G163K+L227F+T231R+N233R+Q249R;
D27V+N33Q+G91A+N94R+D111A+G163K+L227F+T231R+N233R+Q249R+D254S;
D27R+N33Q+P136H+L227G+T231R+N233R+Q249R+D254S;
N11R+N33Q+T231R+N233R+T244S;
N33Q+G91T+D96N+D111A+V176I+T231R+N233R+D254S;
N33Q+G91T+N94S+D111A+V176I+T231R+N233R+D254S;
N33Q+G161A+T231R+N233R;
N33Q+G38I+G177A+T231R+N233R;
N33Q+N101Q+T231R+N233R;
N33Q+N94Q+T231R+N233R;
N33Q+G161A+T231R+N233R;
N11Q+N33Q+T231R+N233R;
N8Q+N33Q+T231R+N233R;
N33Q+T231R+N233R+N251Q;
N33Q+N200Q+T231R+N233R;
N33Q+G177A+T231R+N233R;
N33Q+N73Q+T231R+N233R;
N33Q+I86L+T231R+N233R;
N33Q+K98I+G163K+T231R+N233R;
D27R+N33Q+G91T+D96E+D111A+G163K+T231R+N233R+D254S+P256T;
D27R+N33Q+G91T+D96E+D111A+G163A+T231R+N233R+D254S+P256T;
D27R+N33Q+S216P+L227G+T231R+N233R+Q249R;
N33Q+K98I+G163K+N200Q+T231R+N233R+N251S;
N33Q+G38S+G163K+T231R+N233R;
D27R+N33Q+G38A+G91T+D96E+D111A+T231R+N233R+D254S+P256T;
N33Q+G38Y+T231R+N233R;
D27R+N33Q+G91T+N94R+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+G91T+N94R+D111A+S216P+L227G+T231R+N233R+P256T;
N33Q+G38N+N73Q+T231R+N233R;
N33Q+G38D+R84E+T231R+N233R;
N33Q+G38Q+T231R+N233R;
N33Q+G38I+T231R+N233R;
N33Q+G38K+T231R+N233R;
N33Q+G38F+T231R+N233R;
N33Q+G38H+N200Q+T231R+N233R+N251S;
N33Q+G38L+T231R+N233R;
N33Q+G38M+T231R+N233R;
N33Q+G38F+T231R+N233R;
N33Q+G38P+T231R+N233R;
N33Q+G38T+T231R+N233R;
N11R+N33Q+G91T+W117I+G163K+T231R+N233R+D254S;
D27R+N33Q+G38A+G91T+D96E+D111A+G163K+T231R+N233R+D254S+P256T;
N11R+N33Q+G91T+W117I+G163K+T231R+N233R+D254S;
D27R+N33Q+G38A+G91T+D96E+D111A+G163A+T231R+N233R+D254S+P256T;
D27R+N33Q+V176Q+L227G+T231R+N233R+Q249R+D254S;
N33Q+W117I+V176Q+T231R+N233R+P256A;
N33Q+G38A+G163A+T231R+N233R+P256A;
N33Q+W117I+V176Q+T231R+N233R;
N33Q+G177A+T231R+N233R+G246A;
E1N+N33Q+T231R+N233R;
N33Q+G38H+T231R+N233R;
N33Q+G91A+N94K+D111A+G163K+L227F+T231R+N233R+Q249R+D254S;
N11R+N33Q+G91T+G163K+V176Q+T231R+N233R+D254S;
N33Q+K98I+T231R+N233R;
D27R+N33Q+W117I+V176Q+L227G+T231R+N233R+Q249R+D254S;
N11R+N33Q+G38A+G91T+G163K+T231R+N233R+D254S;
N33Q+G163W+T231R+N233R;
N33Q+G38A+G163A+T231R+N233R;
D27R+N33Q+G91T+D96E+L97Q+D111A+T231R+N233R+D254S+P256T;
N33Q+T231R+N233R+D254Q;
N11R+N33Q+G91T+S115L+G163K+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+V176W+T231R+N233R+D254S;
N33Q+G163D+T231R+N233R;
N33Q+G163P+T231R+N233R;
E1D+N33Q+G91T+N94R+D111A+W117L+T231R+N233R+D254S;
N33Q+G91T+N94R+D111A+W117L+V176W+T231R+N233R;
Q4P+D27R+N33Q+G91N+N94R+D111A+L206F+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+T37K+N711+G91N+N94R+K98I+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+E43K+K46M+I90V+G91N+N94R+D111A+T114I+S216P+L227G+T231R+N233R+P256T;
N33Q+W117S+T231R+N233R;
N33Q+G61R+V63R+G156R+V176W+T231R+N233R+P256I;
N33Q+D96N+G156R+V176W+T231R+N233R;
N33Q+G156R+V176W+T231R+N233R+Q249R;
N33Q+G91T+N94S+D111A+G163T+V176W+T231R+N233R;
N33Q+G91T+N94S+D111A+S115L+G163T+V176I+T231R+N233R;
N11R+D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+G163T+S216P+L227G+T231R+N233R+D254S+P256T;
D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+G163T+S216P+L227G+T231R+N233R+D254S+P256T;
N11R+D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+S216P+L227G+T231R+N233R+D254S+P256T;
D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+S216P+L227G+T231R+N233R+D242E+D254S+P256T;
D27R+N33Q+G38A+E56Q+D57N+G91N+N94R+D111S+S216P+L227G+T231R+N233R+D254S+P256T;
Q4R+D27Q+N33Q+G91T+N94S+E99D+D111A+E210D+S216P+L227G+T231R+N233R+P256L;
N33Q+G38A+G91T+G163A+T231R+N233R+D254S;
N33Q+G38A+G163A+T231R+N233R+D254I;
N11R+N33Q+I90L+G163L+T231R+N233R;
N11R+N33Q+I90L+G163L+T231R+N233R+D254S;
N11R+N33Q+E56Q+G91T+G163K+V176Q+T231R+N233R+D254S;
N11R+D27R+N33Q+G91T+D96E+D111A+G163K+T231R+N233R+D254S+P256T;
N11R+N33Q+G38A+G91T+G112A+G163A+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+E210D+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+T231R+N233R+D254I;
N11R+N33Q+G91T+G163K+V176T+T231R+N233R+D254S;
N11R+N33Q+G91T+G163P+T231R+N233R+D254S;
N11R+N33Q+G91M+G163T+T231R+N233R+D254S;
N11R+N33Q+G38A+G91T+G163K+V176D+T231R+N233R+D254S;
N33Q+E56Q+G156R+V176W+T231R+N233R;
E1D+N33Q+G38A+G91T+N94R+D111A+W117L+V176W+T231R+N233R;
N33Q+G163K+G177A+T231R+N233R+G246A;
N11R+N33Q+E56Q+G91T+G163K+T231R+N233R+D254S;
N11R+N33Q+I90L+G163K+T231R+N233R+D254S;
D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+S216P+L227G+T231R+N233R+Q249R+D254S+P256T;
D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+S216P+E219D+L227G+T231R+N233R+D254S+P256T;
N11R+N33Q+I90L+G91T+N94S+D96E+G163K+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+V176I+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+V176Q+T231R+N233R+D254S;
N11R+N33Q+G91T+G163A+V176T+T231R+N233R+D254S;
N11R+N33Q+G91T+G163L+V176I+T231R+N233R+D254S;
N11R+N33Q+G91T+G163L+V176T+T231R+N233R+D254S;
N11R+N33Q+G91T+G163L+T231R+N233R+D254S;
N11R+N33Q+G91T+G163P+T231R+N233R+D254S;
N11R+N33Q+G91T+G163P+V176I+T231R+N233R+D254S;
N11R+N33Q+G91T+G163L+T231R+N233R+D254S+P256N;
D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+G163T+S216P+L227G+T231R+N233R+Q249R+D254S+P256T;
Q4R+D27Q+N33Q+G91T+N94S+E99D+D111A+G163A+E210V+S216P+L227G+T231R+N233R+P256L;
Q4R+D27Q+N33Q+G91T+N94S+E99D+D111A+V176I+E210V+S216P+L227G+T231R+N233R+P256L;
N33Q+E210Y+T231R+N233R+D254Y+I255F;
N33Q+L93F+D102Y+T231R+N233R;
D27R+N33Q+L227G+T231R+N233R+Q249R+D254S;
N11S+N33Q+T231R+N233R;
N11R+N33Q+T231R+N233R;
N33Q+G38A+G91T+G163K+T231R+N233R+D254S;
N33Q+W117Y+V176T+T231R+N233R;
N8L+N11R+N33Q+G91T+G163K+T231R+N233R+D254S;
E1N+N33Q+G38A+G91T+G163P+V176F+T231R+N233R;
N11R+N33Q+G38A+G91T+G163P+V176G+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+T231R+N233R+D254A+P256F;
N11R+N33Q+G91T+G163K+T231R+N233R+P256F;
N11R+N33Q+G91T+G163K+T231R+N233R+D254S+P256F;
N11R+N33Q+G38A+G91T+G156R+G163K+V176T+T231R+N233R+D254S;
N33Q+G91K+D96S+G163T+T231R+N233R+Q249R;
N11R+N33Q+G91T+G163N+T231R+N233R+D254S;
N11R+N33Q+G91T+G163T+T231R+N233R+D254S;
N11R+N33Q+G91T+G163W+T231R+N233R+D254S;
N11R+N33Q+G91K+G163K+T231R+N233R+D254S;
N11R+G23E+N33Q+G91T+G163K+T231R+N233R+D254S;
N11R+N33Q+G91T+V141E+G163K+T231R+N233R+D254S;
N11R+N33Q+L52R+G91T+G163K+T231R+N233R+D254S;
N11R+N33Q+G91T+V141L+G163K+T231R+N233R+D254S;
N11R+N33Q+T37K+G91T+G163K+T231R+N233R+D254S;
N11R+N33Q+A68V+G91T+G163K+T231R+N233R+D254S;
N11R+N33Q+G91T+G163A+V176I+T231R+N233R+D254S;
N11R+N33Q+T37M+G91T+G163P+V176T+T231R+N233R+D254S;
N11R+N33Q+G91T+G163L+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+T231R+N233R+D254S+P256I;
N33Q+G38S+G156R+G163K+V176W+T231R+N233R;
N11R+D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+G163K+S216P+L227G+T231R+N233R+D254S+P256T;
N11R+N33Q+G38A+G91T+G163P+V176G+T231R+N233R+D254S;
N11R+N33Q+G38A+G91T+G163Q+V176G+T231R+N233R+D254S;
N11R+N33Q+G38A+G91T+G163T+V176G+T231R+N233R+D254S;
N11R+N33Q+G38A+G91T+N94R+G163P+V176G+T231R+N233R+D254S;
E1*+N11R+N33Q+G38A+G91N+N94R+G163P+V176G+T231R+N233R+D254S;
E1N+N11R+N33Q+G38A+G91T+G163P+V176F+T231R+N233R;
E1N+F10L+N11R+N33Q+G38A+G91T+G163P+V176F+T231R+N233R;
E1N+N33Q+G38A+G91T+G163P+V176F+T231R+N233R+D254S;
E1N+N33Q+G38A+G91T+D111A+G163P+V176F+T231R+N233R;
E1N+N33Q+G38A+G91T+G163P+V176F+L227F+T231R+N233R;
E1N+N11R+N33Q+G38A+G91T+D111A+G163P+V176F+T231R+N233R;
E1N+N33Q+G38A+G91T+G163P+V176F+L227F+T231R+N233R+D254S;
E1N+N33Q+G38A+G91T+G163P+V176F+T231R+N233R+D254S+I255A+P256Q;
E1N+N11R+N33Q+G38A+G91T+D111A+G163P+V176F+T231R+N233R+D254S;
N33Q+G156R+V176W+T231R+N233R+P256I;
N33Q+G91T+N94S+D111A+G156R+G163T+V176W+T231R+N233R;
N33Q+G91T+N94S+D111A+G156R+G163T+V176I+T231R+N233R;
N11R+N33Q+G38A+G91T+D102G+S115L+G163K+T231R+N233R+D254S+P256T;
N11R+N33Q+G38A+G91T+S115L+G163K+T231R+N233R+D254S+P256T;
E1N+N11R+N33Q+G91T+G163A+T231R+N233R+G246A+D254S;
N11R+D27R+N33Q+D57G+G91T+D96E+D111A+G163K+T231R+N233R+D254S+P256T;
N33Q+D96N+G156R+V176W+T231R+N233R+Q249R;
N33Q+I86F+L93F+D102Y+E210Y+L227F+T231R+N233R+D254Y+I255F+L269F;
N33Q+I86F+L93F+D102Y+E210Y+L227F+T231R+N233R+D254Y+I255F;
N11C+N33Q+G91T+G163K+T231R+N233R+D254S;
N11L+N33Q+G91T+G163K+T231R+N233R+D254S;
N11H+N33Q+G91T+G163K+T231R+N233R+D254S;
N11D+N33Q+G91T+G163K+T231R+N233R+D254S;
N11R+N33Q+G91T+D96W+G163K+T231R+N233R+D254S;
D27R+N33Q+G91T+D96E+L97Q+D111A+G163K+T231R+N233R+D254S+P256T;
N11P+N33Q+G91T+G163K+T231R+N233R+D254S;
Q4R+D27N+N33Q+G38A+G91T+N94S+E99D+D111A+V176I+E210V+S216P+L227G+T231R+N233R+P256L;
N11R+N33Q+E56Q+G163K+T231R+N233R+D254S;
N11R+N33Q+G91T+G163A+T231R+N233R+D254S;
N11R+N33Q+G91T+G163P+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+L227G+P229R+T231R+N233R+D254S;
N33Q+E87K+T231R+N233R;
N33Q+N94K+T231R+N233R;
N33Q+D96Y+T231R+N233R;
N33Q+K98I+T231R+N233R;
A30V+N33Q+K98I+T231R+N233R;
N33Q+E87K+D96E+T231R+N233R;
N26I+N33Q+T231R+N233R;
A30T+N33Q+T231R+N233R;
N33Q+G91V+T231R+N233R;
N33Q+G91A+T231R+N233R;
N33Q+G91V+L97M+T231R+N233R;
N33Q+K98I+T231R+N233R;
N33Q+L69I+G91E+T231R+N233R;
P29T+N33Q+T231R+N233R;
N33Q+G91V+T231R+N233R;
N33Q+K98I+T231R+N233R;
N33Q+G91E+T231R+N233R;
N33Q+N94K+T231R+N233R;
D27R+N33Q+G91N+N94R+K98I+D111A+N162S+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+T37K+N711+G91N+N94R+K98I+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+N39S+G91N+N94R+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+I76T+G91N+N94R+R108M+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+L52I+V60E+G91N+N94R+D111A+T114I+V168M+E210D+S216P+L227G+T231R+N233R+P256T;
Q4P+D27R+N33Q+G91N+N94R+D111A+R205I+L206F+S216P+L227G+T231R+N233R+P256T;
Q4H+D27R+N33Q+G91N+N94R+D111A+V154L+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+G91N+N94R+D111A+V154I+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+N71S+G91N+N94R+D111A+H135D+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+G91N+N94R+K98I+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+G91N+N94R+L97M+D111A+S216P+T226N+L227G+T231R+N233R+P256T+L269H;
D27R+N33Q+G91N+N94R+D111A+T114I+R179T+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+G91N+N94R+D111A+S216P+L227G+T231R+N233R；
G23E+D27R+N33Q+L52R+G91N+N94R+D111A+T114I+V141E+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+E43K+K46M+I90V+G91N+N94R+D111A+T114I+S216P+L227G+T231R+N233R+P256T;
D27R+A30V+N33Q+G91N+N94R+G109A+D111A+G190D+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+A49T+G91N+N94R+D111A+Y138F+G163R+S216P+L227G+T231R+N233R+P256T;
N26H+D27R+N33Q+G91N+N94R+D111A+V154F+G190C+S216P+L227G+T231R+N233R+P256T;
N33Q+G91T+D96E+K98T+T114I+G163S+E210V+T231R+N233R+D254K+P256A;
N33Q+G91T+D96E+K98T+T114I+T231R+N233R+G163S;
N33Q+G91T+D96E+K98T+T114I+G163K+E210D+T231R+N233R;
N33Q+G91T+T114I+G163K+E210D+T231R+N233R+D254G+P256A;
D27R+N33Q+G91T+T114I+G163W+E210D+T231R+N233R;
D27N+N33Q+G91T+T114I+G163S+E210D+T231R+N233R+P256T;
N33Q+G91T+T114I+G163K+E210D+T231R+N233R;
N33Q+G38W+G91T+T114I+G163K+E210V+T231R+N233R;
N33Q+G38W+G91T+T114I+G163K+E210D+T231R+N233R+P256T;
D27I+N33Q+G91T+D96E+K98T+T114I+G163K+E210D+T231R+N233R+P256T;
N33Q+G91T+T114I+E210V+T231R+N233R+D254K+P256A;
N33Q+G91A+N94K+D111A+G163K+L227F+T231R+N233R+Q249R;
G23E+D27R+N33Q+L52R+G91N+N94R+D111A+T114I+V141E+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+E43K+K46M+I90V+G91N+N94R+D111A+T114I+S216P+L227G+T231R+N233R+P256T;
N33Q+G91T+K98I+T114I+G163K+T231R+N233R+D254S;
N33Q+G91T+K98I+G163K+T231R+N233R+D254S+P256L;
N33Q+G91T+T114I+G163K+T231R+N233R+D254S+P256L;
G23E+D27R+N33Q+L52R+G91N+N94R+D111A+T114I+V141E+S216P+L227G+T231R+N233R+P256T; 及び
D27R+N33Q+E43K+K46M+I90V+G91N+N94R+D111A+T114I+S216P+L227G+T231R+N233R+P256T、
から選択される置換組を含んで成る請求項１〜２及び５のいずれか１項記載のリパーゼ。
【請求項７】
（ａ）配列番号２のアミノ酸１〜269の配列に対して少なくとも50％の同一性を有し；
（ｂ）リパーゼ活性を有し；そして
（ｃ）配列番号2のアミノ酸１〜269の配列に比較して、次の位置：9, 30, 38, 39, 63, 112, 114, 115, 117, 122, 128, 130, 136, 154, 155, 156, 161 , 163, 168, 185, 190, 239, 及び246から選択された少なくとも１つの位置で置換を含んで成るリパーゼ。
【請求項８】
（ａ）配列番号２のアミノ酸１〜269の配列に対して少なくとも50％の同一性を有し；
（ｂ）リパーゼ活性を有し；そして
（ｃ）配列番号2のアミノ酸１〜269の配列に比較して、次の置換：E1N;Q4H;N8L,Q;Q9H;N11C,D,H,L,P,S; G23E; D27I; P29T; A30T,V; T37K,M; G38A,D,F,H,I,K,L,M,N,P,Q,S,T,W,Y; N39H,S; D57N; G61R; V63R; N71I,S; N73Q,Y; I76T; I86F,L; E87H; G91F,K,L,M,V,Y; N94Q; F95*; D96*; N101Q; D111E; G112A; T114I; S115L; W117C,D,E,F,G,H,I,K,L,P,S,T,V,Y; D122E,N; Q126L; V128A, D130H, H135D, P136H; V141E,L;V154F,I,L;A155V;G156R;G161A,E;N162G,S;G163A,C,D,E,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y; V168M; L185M; G190C,D; R205I; G240L; G246A; N251Q,S;及びL269F,Hから選択された少なくとも１つの置換を含んで成るリパーゼ。
【請求項９】
（ａ）配列番号２のアミノ酸１〜269の配列に対して少なくとも50％の同一性を有し；
（ｂ）リパーゼ活性を有し；そして
（ｃ）配列番号2のアミノ酸１〜269の配列に比較して、次の置換：
D27R+N33Q+G91A+D96E+L97Q+D111A+T231R+N233R+P256T;
N33Q+E210D+T231R+N233R;
N33Q+D111A+T231R+N233R;
N33Q+G91T+T231R+N233R;
N33Q+E219D+T231R+N233R;
N33Q+W117L+T231R+N233R;
D27Q+N33Q+T231R+N233R;
N33Q+G91T+T231R+N233R;
D27S+N33Q+G91A+D96E+L97Q+D111A+S216P+T231R+N233R+P256T;
D27R+N33Q+G91N+N94R+D111A+T231R+N233R+P256T;
D27R+N33Q+G91T+N94S+D111A+S216P+L227G+T231R+N233R+P256T;
Q4R+N33Q+T231R+N233R;
N33Q+T231R+N233R+Q249R;
N33Q+D96W+T231R+N233R;
D27V+N33Q+V60S+D96W+T231R+N233R+Q249R;
D27V+N33Q+V60S+T231R+N233R+Q249R;
Q9H+N33Q+D102E+T231R+N233R;
N33Q+D111E+T231R+N233R;
N33Q+D122E+T231R+N233R;
D27R+N33Q+G91N+N94R+D111A+S216P+L227G+T231R+N233R+P256T;
N33Q+D167E+T231R+N233R;
N33Q+G91N+T231R+N233R;
N33Q+T231R+N233R+P256T;
D27R+N33Q+G91A+L93*+N94*+F95*+D96*+D111A+T231R+N233R+P256T;
N11R+N33Q+T231R+N233R;
N33Q+N39H+T231R+N233R;
N33Q+P229R+T231R+N233R;
D27R+N33Q+G91N+N94R+D111A+G163K+S216P+L227G+T231R+N233R+P256T;
N33Q+G91T+G163K+T231R+N233R;
D27R+N33Q+G91A+D96E+L97Q+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+G91A+D96E+L97Q+D111A+S216P+T231R+N233R+P256T;
N33Q+E87A+T231R+N233R;
N33Q+E56Q+T231R+N233R;
N33Q+E210V+T231R+N233R;
N33Q+E56K+T231R+N233R;
N33Q+T231R+N233R+D254G;
N33Q+D96S+T231R+N233R;
N33Q+D122N+T231R+N233R;
N26A+N33Q+T231R+N233R;
N33Q+N162T+T231R+N233R;
N33Q+A150V+N162G+T231R+N233R;
N33Q+I90L+G163L+T231R+N233R;
N33Q+T231R+N233R+G240L;
D27R+N33Q+G91A+D96E+D111A+T231R+N233R+D254G+P256T;
D27R+N33Q+G91A+N94S+D111A+T231R+N233R+P256T;
N33Q+N200S+T231R+N233R;
N33Q+N39S+T231R+N233R;
N33Q+E210R+T231R+N233R;
N33Q+N39H+T231R+N233R+D254R;
N33Q+T231R+N233R+D254R;
N33Q+N94R+T231R+N233R;
N33Q+D96R+T231R+N233R;
D27N+N33Q+T231R+N233R;
D27N+N33Q+E56R+T231R+N233R;
N33Q+L227F+T231R+N233R;
N33Q+N73Y+G225P+T231R+N233R;
N33Q+G225P+T231 R+N233R;
N33Q+T231R+N233R+D254S;
N33Q+D96G+T231R+N233R;
N33Q+D96N+T231R+N233R+D254S;
N33Q+T231R+N233R+D254G;
N33Q+D130H+T231R+N233R;
N33Q+E87A+T231R+N233R;
N33Q+T231R+N233R+E239D;
N33Q+D111A+T231R+N233R+D254G;
N33Q+E210V+T231R+N233R+D254S;
N11R+N33Q+E210V+T231R+N233R+D254S;
N33Q+G91T+G163K+T231R+N233R+D254G;
N33Q+G91T+G163K+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+T231R+N233R+D254S;
Q4R+D27R+N33Q+G91T+N94S+D111A+S216P+L227G+T231R+N233R+P256T;
N33Q+G91T+N94S+D111A+V176I+T231R+N233R;
Q4R+D27R+N33Q+G91T+N94S+D111A+E210D+S216P+L227G+T231R+N233R+P256T;
Q4R+D27Q+N33Q+G91T+N94S+D111A+S216P+L227G+T231R+N233R+P256T;
N33Q+G91T+N94S+D111A+T231R+N233R+P256T;
N33Q+G177A+T231R+N233R;
N33Q+T231R+N233R+G246A;
D27N+N33Q+G91T+G163K+T231R+N233R+D254S;
D27Q+N33Q+G91T+G163K+E219D+T231R+N233R;
N33Q+G91T+E219D+T231R+N233R;
K98I+T231R+N233R+N251S;
N33Q+G163R+T231R+N233R;
N33Q+G163N+T231R+N233R;
N33Q+G163C+T231R+N233R;
N33Q+G163Q+T231R+N233R;
N33Q+G163E+T231R+N233R;
N33Q+G163H+T231R+N233R;
N33Q+G163I+T231R+N233R;
N33Q+G163P+T231R+N233R;
N33Q+G163D+T231R+N233R;
N33Q+G91K+T231R+N233R;
N33Q+G91M+T231R+N233R;
N33Q+G91F+T231R+N233R;
N33Q+G91S+T231R+N233R;
N33Q+G91W+T231R+N233R;
N33Q+G91Y+T231R+N233R;
N33Q+G163T+T231R+N233R;
N33Q+G163W+T231R+N233R;
N33Q+G163Y+T231R+N233R;
N33Q+G163V+T231R+N233R;
N33Q+G91C+T231R+N233R;
N33Q+G91Y+Q126L+T231R+N233R;
N33Q+G91M+G161E+T231R+N233R;
N33Q+V128A+T231R+N233R;
N33Q+G163V+L185M+T231R+N233R;
N33Q+G38A+T231R+N233R;
N33Q+G163A+T231R+N233R;
N33Q+G91T+N94S+D111A+T231R+N233R;
N33Q+G38A+G163A+T231R+N233R;
N33Q+G163M+T231R+N233R;
N33Q+G91V+T231R+N233R;
N33Q+D111A+T231R+N233R+Q249R;
N33Q+D111A+T231R+N233R+D254S;
D27R+N33Q+G91A+D96E+L97Q+D111A+T231R+N233R+D254S+P256T;
D27R+N33Q+G91A+D96E+L97Q+D111A+T231R+N233R+D254G+P256T;
N33Q+G91T+N94R+T231R+N233R+D254S;
N33Q+G91T+N94R+D111A+W117L+T231R+N233R;
N33Q+W117L+T231R+N233R+D254S;
N33Q+T231R+N233R+P256T;
N33Q+T231R+N233R+D242E;
N33Q+E87R+T231R+N233R;
N33Q+E56R+T231R+N233R;
N33Q+N162G+T231R+N233R;
N33Q+G91L+T231R+N233R;
N33Q+E87H+T231R+N233R;
N33Q+D96N+T231R+N233R+Q249R;
N33Q+G91T+N94R+T231R+N233R+D254S;
N33Q+L227F+T231R+N233R+D254S;
D27R+N33Q+G91T+D96E+L97Q+D111A+T231R+N233R+D254S+P256T;
N33Q+G163A+T231R+N233R;
D27R+N33Q+G91T+D96E+D111A+T231R+N233R+D254S+P256T;
N33Q+G91T+N94R+T231R+N233R;
N33Q+T231R+N233R+D254A;
N33Q+T231R+N233R+D254N;
N33Q+T231R+N233R+D254Q;
N33Q+T231R+N233R+D254I;
N33Q+T231R+N233R+D254L;
N33Q+T231R+N233R+D254K;
N33Q+T231R+N233R+D254M;
N33Q+S216P+L227G+T231R+N233R+Q249R;
D27V+N33Q+V60S+G91T+D96W+T231R+N233R+Q249R;
N33Q+D96N+L227G+T231R+N233R+Q249R;
D27R+N33Q+L227G+T231R+N233R;
D27R+N33Q+L227G+T231R+N233R+Q249R;
N33Q+E219D+L227G+T231R+N233R+Q249R;
D27Q+N33Q+L227G+T231R+N233R+Q249R;
N33Q+W117L+L227G+T231R+N233R+Q249R;
D5E+N33Q+W117L+L227G+T231R+N233R+Q249R;
D27Q+N33Q+E219D+L227G+T231R+N233R+Q249R;
N33Q+D96E+E219D+L227G+T231R+N233R+Q249R;
D27R+N33Q+E56K+G91N+N94R+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+E56Q+D57N+G91N+N94R+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+S216P+L227G+T231R+N233R+D254S+P256T;
D27R+N33Q+E56S+G91N+N94R+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+G91N+N94R+D111A+S216P+L227G+T231R+N233R+D254S+P256T;
D27R+N33Q+G91N+N94R+D111A+S216P+L227G+T231R+N233R+D254S+P256T;
D27R+N33Q+G91N+N94R+D111S+A155V+S216P+L227G+T231R+N233R+D254S+P256T;
D27R+N33Q+G91N+N94R+D111S+S216P+L227G+T231R+N233R+D254S+P256T;
N33Q+D111A+T231R+N233R+D254S;
N33Q+D111A+W117L+T231R+N233R+D254S;
N33Q+T231R+N233R+P256A;
N33Q+T231R+N233R+P256N;
N33Q+T231R+N233R+P256G;
N33Q+T231R+N233R+P256H;
N33Q+T231R+N233R+P256L;
N33Q+T231R+N233R+P256M;
N33Q+T231R+N233R+P256S;
N33Q+T231R+N233R+P256W;
N33Q+T231R+N233R+P256Y;
N33Q+T231R+N233R+P256F;
N33Q+T231R+N233R+P256V;
N33Q+G91M+G163W+T231R+N233R;
N33Q+G91M+G163T+T231R+N233R;
N33Q+G91M+G163D+T231R+N233R;
N33Q+G91K+G163W+T231R+N233R;
N33Q+G91T+G163W+T231R+N233R;
N33Q+V176N+T231R+N233R;
N33Q+V176D+T231R+N233R;
N33Q+W117F+T231R+N233R;
N33Q+G91T+N94S+D111A+V176I+T231R+N233R+D254S;
N33Q+V176I+T231R+N233R;
N33Q+D111N+T231R+N233R;
N33Q+D111N+G225P+T231 R+N233R;
N33Q+D111N+S216P+T231R+N233R;
D27R+N33Q+G91T+N94R+D111A+S216P+L227G+T231R+N233R;
N33Q+G91M+G163P+T231R+N233R;
N33Q+G91T+G163A+T231R+N233R;
N33Q+W117D+T231R+N233R;
N33Q+W117H+T231R+N233R;
N33Q+W117C+T231R+N233R;
N33Q+W117K+T231R+N233R;
N33Q+W117V+T231R+N233R;
N11S+N33Q+T231R+N233R;
N33Q+W117E+V176K+T231R+N233R;
N33Q+W117G+T231R+N233R;
N33Q+W117P+T231R+N233R;
N33Q+W117S+T231R+N233R;
N33Q+W117T+T231R+N233R;
N33Q+W117I+T231R+N233R;
D27R+N33Q+L227G+T231R+N233R+Q249R+D254S;
N33Q+S115L+T231R+N233R;
N33Q+G38A+G91T+G163K+T231R+N233R+D254S;
N33Q+V176M+T231R+N233R;
N33Q+V176H+T231R+N233R;
N33Q+V176A+T231R+N233R;
D27V+N33Q+L227F+T231R+N233R+Q249R;
N33Q+W117Y+T231R+N233R;
N33Q+W117Y+V176D+T231R+N233R;
D27V+N33Q+G91A+N94R+D111A+G163K+L227F+T231R+N233R+Q249R;
D27V+N33Q+G91A+N94R+D111A+G163K+L227F+T231R+N233R+Q249R+D254S;
D27R+N33Q+P136H+L227G+T231R+N233R+Q249R+D254S;
N11R+N33Q+T231R+N233R+T244S;
N33Q+G91T+D96N+D111A+V176I+T231R+N233R+D254S;
N33Q+G91T+N94S+D111A+V176I+T231R+N233R+D254S;
N33Q+G161A+T231R+N233R;
N33Q+G38I+G177A+T231R+N233R;
N33Q+N101Q+T231R+N233R;
N33Q+N94Q+T231R+N233R;
N33Q+G161A+T231R+N233R;
N11Q+N33Q+T231R+N233R;
N8Q+N33Q+T231R+N233R;
N33Q+T231R+N233R+N251Q;
N33Q+N200Q+T231R+N233R;
N33Q+G177A+T231R+N233R;
N33Q+N73Q+T231R+N233R;
N33Q+I86L+T231R+N233R;
N33Q+K98I+G163K+T231R+N233R;
D27R+N33Q+G91T+D96E+D111A+G163K+T231R+N233R+D254S+P256T;
D27R+N33Q+G91T+D96E+D111A+G163A+T231R+N233R+D254S+P256T;
D27R+N33Q+S216P+L227G+T231R+N233R+Q249R;
N33Q+K98I+G163K+N200Q+T231R+N233R+N251S;
N33Q+G38S+G163K+T231R+N233R;
D27R+N33Q+G38A+G91T+D96E+D111A+T231R+N233R+D254S+P256T;
N33Q+G38Y+T231R+N233R;
D27R+N33Q+G91T+N94R+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+G91T+N94R+D111A+S216P+L227G+T231R+N233R+P256T;
N33Q+G38N+N73Q+T231R+N233R;
N33Q+G38D+R84E+T231R+N233R;
N33Q+G38Q+T231R+N233R;
N33Q+G38I+T231R+N233R;
N33Q+G38K+T231R+N233R;
N33Q+G38F+T231R+N233R;
N33Q+G38H+N200Q+T231R+N233R+N251S;
N33Q+G38L+T231R+N233R;
N33Q+G38M+T231R+N233R;
N33Q+G38F+T231R+N233R;
N33Q+G38P+T231R+N233R;
N33Q+G38T+T231R+N233R;
N11R+N33Q+G91T+W117I+G163K+T231R+N233R+D254S;
D27R+N33Q+G38A+G91T+D96E+D111A+G163K+T231R+N233R+D254S+P256T;
N11R+N33Q+G91T+W117I+G163K+T231R+N233R+D254S;
D27R+N33Q+G38A+G91T+D96E+D111A+G163A+T231R+N233R+D254S+P256T;
D27R+N33Q+V176Q+L227G+T231R+N233R+Q249R+D254S;
N33Q+W117I+V176Q+T231R+N233R+P256A;
N33Q+G38A+G163A+T231R+N233R+P256A;
N33Q+W117I+V176Q+T231R+N233R;
N33Q+G177A+T231R+N233R+G246A;
E1N+N33Q+T231R+N233R;
N33Q+G38H+T231R+N233R;
N33Q+G91A+N94K+D111A+G163K+L227F+T231R+N233R+Q249R+D254S;
N11R+N33Q+G91T+G163K+V176Q+T231R+N233R+D254S;
N33Q+K98I+T231R+N233R;
D27R+N33Q+W117I+V176Q+L227G+T231R+N233R+Q249R+D254S;
N11R+N33Q+G38A+G91T+G163K+T231R+N233R+D254S;
N33Q+G163W+T231R+N233R;
N33Q+G38A+G163A+T231R+N233R;
D27R+N33Q+G91T+D96E+L97Q+D111A+T231R+N233R+D254S+P256T;
N33Q+T231R+N233R+D254Q;
N11R+N33Q+G91T+S115L+G163K+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+V176W+T231R+N233R+D254S;
N33Q+G163D+T231R+N233R;
N33Q+G163P+T231R+N233R;
E1D+N33Q+G91T+N94R+D111A+W117L+T231R+N233R+D254S;
N33Q+G91T+N94R+D111A+W117L+V176W+T231R+N233R;
Q4P+D27R+N33Q+G91N+N94R+D111A+L206F+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+T37K+N711+G91N+N94R+K98I+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+E43K+K46M+I90V+G91N+N94R+D111A+T114I+S216P+L227G+T231R+N233R+P256T;
N33Q+W117S+T231R+N233R;
N33Q+G61R+V63R+G156R+V176W+T231R+N233R+P256I;
N33Q+D96N+G156R+V176W+T231R+N233R;
N33Q+G156R+V176W+T231R+N233R+Q249R;
N33Q+G91T+N94S+D111A+G163T+V176W+T231R+N233R;
N33Q+G91T+N94S+D111A+S115L+G163T+V176I+T231R+N233R;
N11R+D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+G163T+S216P+L227G+T231R+N233R+D254S+P256T;
D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+G163T+S216P+L227G+T231R+N233R+D254S+P256T;
N11R+D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+S216P+L227G+T231R+N233R+D254S+P256T;
D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+S216P+L227G+T231R+N233R+D242E+D254S+P256T;
D27R+N33Q+G38A+E56Q+D57N+G91N+N94R+D111S+S216P+L227G+T231R+N233R+D254S+P256T;
Q4R+D27Q+N33Q+G91T+N94S+E99D+D111A+E210D+S216P+L227G+T231R+N233R+P256L;
N33Q+G38A+G91T+G163A+T231R+N233R+D254S;
N33Q+G38A+G163A+T231R+N233R+D254I;
N11R+N33Q+I90L+G163L+T231R+N233R;
N11R+N33Q+I90L+G163L+T231R+N233R+D254S;
N11R+N33Q+E56Q+G91T+G163K+V176Q+T231R+N233R+D254S;
N11R+D27R+N33Q+G91T+D96E+D111A+G163K+T231R+N233R+D254S+P256T;
N11R+N33Q+G38A+G91T+G112A+G163A+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+E210D+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+T231R+N233R+D254I;
N11R+N33Q+G91T+G163K+V176T+T231R+N233R+D254S;
N11R+N33Q+G91T+G163P+T231R+N233R+D254S;
N11R+N33Q+G91M+G163T+T231R+N233R+D254S;
N11R+N33Q+G38A+G91T+G163K+V176D+T231R+N233R+D254S;
N33Q+E56Q+G156R+V176W+T231R+N233R;
E1D+N33Q+G38A+G91T+N94R+D111A+W117L+V176W+T231R+N233R;
N33Q+G163K+G177A+T231R+N233R+G246A;
N11R+N33Q+E56Q+G91T+G163K+T231R+N233R+D254S;
N11R+N33Q+I90L+G163K+T231R+N233R+D254S;
D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+S216P+L227G+T231R+N233R+Q249R+D254S+P256T;
D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+S216P+E219D+L227G+T231R+N233R+D254S+P256T;
N11R+N33Q+I90L+G91T+N94S+D96E+G163K+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+V176I+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+V176Q+T231R+N233R+D254S;
N11R+N33Q+G91T+G163A+V176T+T231R+N233R+D254S;
N11R+N33Q+G91T+G163L+V176I+T231R+N233R+D254S;
N11R+N33Q+G91T+G163L+V176T+T231R+N233R+D254S;
N11R+N33Q+G91T+G163L+T231R+N233R+D254S;
N11R+N33Q+G91T+G163P+T231R+N233R+D254S;
N11R+N33Q+G91T+G163P+V176I+T231R+N233R+D254S;
N11R+N33Q+G91T+G163L+T231R+N233R+D254S+P256N;
D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+G163T+S216P+L227G+T231R+N233R+Q249R+D254S+P256T;
Q4R+D27Q+N33Q+G91T+N94S+E99D+D111A+G163A+E210V+S216P+L227G+T231R+N233R+P256L;
Q4R+D27Q+N33Q+G91T+N94S+E99D+D111A+V176I+E210V+S216P+L227G+T231R+N233R+P256L;
N33Q+E210Y+T231R+N233R+D254Y+I255F;
N33Q+L93F+D102Y+T231R+N233R;
D27R+N33Q+L227G+T231R+N233R+Q249R+D254S;
N11S+N33Q+T231R+N233R;
N11R+N33Q+T231R+N233R;
N33Q+G38A+G91T+G163K+T231R+N233R+D254S;
N33Q+W117Y+V176T+T231R+N233R;
N8L+N11R+N33Q+G91T+G163K+T231R+N233R+D254S;
E1N+N33Q+G38A+G91T+G163P+V176F+T231R+N233R;
N11R+N33Q+G38A+G91T+G163P+V176G+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+T231R+N233R+D254A+P256F;
N11R+N33Q+G91T+G163K+T231R+N233R+P256F;
N11R+N33Q+G91T+G163K+T231R+N233R+D254S+P256F;
N11R+N33Q+G38A+G91T+G156R+G163K+V176T+T231 R+N233R+D254S;
N33Q+G91K+D96S+G163T+T231R+N233R+Q249R;
N11R+N33Q+G91T+G163N+T231R+N233R+D254S;
N11R+N33Q+G91T+G163T+T231R+N233R+D254S;
N11R+N33Q+G91T+G163W+T231R+N233R+D254S;
N11R+N33Q+G91K+G163K+T231R+N233R+D254S;
N11R+G23E+N33Q+G91T+G163K+T231R+N233R+D254S;
N11R+N33Q+G91T+V141 E+G163K+T231R+N233R+D254S;
N11R+N33Q+L52R+G91T+G163K+T231R+N233R+D254S;
N11R+N33Q+G91T+V141L+G163K+T231R+N233R+D254S;
N11R+N33Q+T37K+G91T+G163K+T231R+N233R+D254S;
N11R+N33Q+A68V+G91T+G163K+T231 R+N233R+D254S;
N11R+N33Q+G91T+G163A+V176I+T231R+N233R+D254S;
N11R+N33Q+T37M+G91T+G163P+V176T+T231R+N233R+D254S;
N11R+N33Q+G91T+G163L+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+T231R+N233R+D254S+P256I;
N33Q+G38S+G156R+G163K+V176W+T231R+N233R;
N11R+D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+G163K+S216P+L227G+T231R+N233R+D254S+P256T;
N11R+N33Q+G38A+G91T+G163P+V176G+T231R+N233R+D254S;
N11R+N33Q+G38A+G91T+G163Q+V176G+T231R+N233R+D254S;
N11R+N33Q+G38A+G91T+G163T+V176G+T231R+N233R+D254S;
N11R+N33Q+G38A+G91T+N94R+G163P+V176G+T231R+N233R+D254S;
E1*+N11R+N33Q+G38A+G91N+N94R+G 163P+ V176G+T231R+N233R+D254S;
E1N+N11R+N33Q+G38A+G91T+G163P+V176F+T231R+N233R;
E1N+F10L+N11R+N33Q+G38A+G91T+G163P+V176F+T231 R+N233R;
E1N+N33Q+G38A+G91T+G163P+V176F+T231R+N233R+D254S;
E1N+N33Q+G38A+G91T+D111A+G163P+V176F+T231 R+N233R;
E1N+N33Q+G38A+G91T+G163P+V176F+L227F+T231R+N233R;
E1N+N11R+N33Q+G38A+G91T+D111A+G163P+V176F+T231R+N233R;
E1N+N33Q+G38A+G91T+G163P+V176F+L227F+T231R+N233R+D254S;
E1N+N33Q+G38A+G91T+G163P+V176F+T231R+N233R+D254S+I255A+P256Q;
E1N+N11R+N33Q+G38A+G91T+D111A+G163P+V176F+T231R+N233R+D254S;
N33Q+G156R+V176W+T231R+N233R+P256I;
N33Q+G91T+N94S+D111A+G156R+G163T+V176W+T231R+N233R;
N33Q+G91T+N94S+D111A+G156R+G163T+V176I+T231R+N233R;
N11R+N33Q+G38A+G91T+D102G+S115L+G163K+T231R+N233R+D254S+P256T;
N11R+N33Q+G38A+G91T+S115L+G163K+T231R+N233R+D254S+P256T;
E1N+N11R+N33Q+G91T+G163A+T231R+N233R+G246A+D254S;
N11R+D27R+N33Q+D57G+G91T+D96E+D111A+G163K+T231R+N233R+D254S+P256T;
N33Q+D96N+G156R+V176W+T231R+N233R+Q249R;
N33Q+I86F+L93F+D102Y+E210Y+L227F+T231R+N233R+D254Y+I255F+L269F;
N33Q+I86F+L93F+D102Y+E210Y+L227F+T231R+N233R+D254Y+I255F;
N11C+N33Q+G91T+G163K+T231R+N233R+D254S;
N11L+N33Q+G91T+G163K+T231R+N233R+D254S;
N11H+N33Q+G91T+G163K+T231R+N233R+D254S;
N11D+N33Q+G91T+G163K+T231R+N233R+D254S;
N11R+N33Q+G91T+D96W+G163K+T231R+N233R+D254S;
D27R+N33Q+G91T+D96E+L97Q+D111A+G163K+T231R+N233R+D254S+P256T;
N11P+N33Q+G91T+G163K+T231R+N233R+D254S;
Q4R+D27N+N33Q+G38A+G91T+N94S+E99D+D111A+V176I+E210V+S216P+L227G+T231R+N233R+P256L;
N11R+N33Q+E56Q+G163K+T231R+N233R+D254S;
N11R+N33Q+G91T+G163A+T231R+N233R+D254S;
N11R+N33Q+G91T+G163P+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+L227G+P229R+T231R+N233R+D254S;
N33Q+E87K+T231R+N233R;
N33Q+N94K+T231R+N233R;
N33Q+D96Y+T231R+N233R;
N33Q+K98I+T231R+N233R;
A30V+N33Q+K98I+T231R+N233R;
N33Q+E87K+D96E+T231R+N233R;
N26I+N33Q+T231R+N233R;
A30T+N33Q+T231R+N233R;
N33Q+G91V+T231R+N233R;
N33Q+G91A+T231R+N233R;
N33Q+G91V+L97M+T231R+N233R;
N33Q+K98I+T231R+N233R;
N33Q+L69I+G91E+T231R+N233R;
P29T+N33Q+T231R+N233R;
N33Q+G91V+T231R+N233R;
N33Q+K98I+T231R+N233R;
N33Q+G91E+T231R+N233R;
N33Q+N94K+T231R+N233R;
D27R+N33Q+G91N+N94R+K98I+D111A+N162S+S216P+L227G+T231R+ N233R+P256T;
D27R+N33Q+T37K+N711+G91N+N94R+K98I+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+N39S+G91N+N94R+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+I76T+G91N+N94R+R108M+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+L52I+V60E+G91N+N94R+D111A+T114I+V168M+E210D+S216P+L227G+T231R+N233R+P256T;
Q4P+D27R+N33Q+G91N+N94R+D111A+R205I+L206F+S216P+L227G+T231R+N233R+P256T;
Q4H+D27R+N33Q+G91N+N94R+D111A+V154L+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+G91N+N94R+D111A+V154I+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+N71S+G91N+N94R+D111A+H135D+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+G91N+N94R+K98I+D111A+S216P+L227G+T231 R+N233R+P256T;
D27R+N33Q+G91N+N94R+L97M+D111A+S216P+T226N+L227G+T231R+N233R+P256T+L269H;
D27R+N33Q+G91N+N94R+D111A+T114I+R179T+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+G91N+N94R+D111A+S216P+L227G+T231R+N233R；
G23E+D27R+N33Q+L52R+G91N+N94R+D111A+T114I+V141E+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+E43K+K46M+I90V+G91N+N94R+D111A+T114I+S216P+L227G+T231R+N233R+P256T;
D27R+A30V+N33Q+G91N+N94R+G109A+D111A+G190D+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+A49T+G91N+N94R+D111A+Y138F+G163R+S216P+L227G+T231R+N233R+P256T;
N26H+D27R+N33Q+G91N+N94R+D111A+V154F+G190C+S216P+L227G+T231R+N233R+P256T;
N33Q+G91T+D96E+K98T+T114I+G163S+E210V+T231R+N233R+D254K+P256A;
N33Q+G91T+D96E+K98T+T114I+T231R+N233R+G163S;
N33Q+G91T+D96E+K98T+T114I+G163K+E210D+T231R+N233R;
N33Q+G91T+T114I+G163K+E210D+T231R+N233R+D254G+P256A;
D27R+N33Q+G91T+T114I+G163W+E210D+T231R+N233R;
D27N+N33Q+G91T+T114I+G163S+E210D+T231R+N233R+P256T;
N33Q+G91T+T114I+G163K+E210D+T231R+N233R;
N33Q+G38W+G91T+T114I+G163K+E210V+T231R+N233R;
N33Q+G38W+G91T+T114I+G163K+E210D+T231R+N233R+P256T;
D27I+N33Q+G91T+D96E+K98T+T114I+G163K+E210D+T231R+N233R+P256T;
N33Q+G91T+T114I+E210V+T231R+N233R+D254K+P256A;
N33Q+G91A+N94K+D111A+G163K+L227F+T231R+N233R+Q249R;
G23E+D27R+N33Q+L52R+G91N+N94R+D111A+T114I+V141E+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+E43K+K46M+I90V+G91N+N94R+D111A+T114I+S216P+L227G+T231R+N233R+P256T;
N33Q+G91T+K98I+T114I+G163K+T231R+N233R+D254S;
N33Q+G91T+K98I+G163K+T231R+N233R+D254S+P256L;
N33Q+G91T+T114I+G163K+T231R+N233R+D254S+P256L;
G23E+D27R+N33Q+L52R+G91N+N94R+D111A+T114I+V141E+S216P+L227G+T231R+N233R+P256T; 及び
D27R+N33Q+E43K+K46M+I90V+G91N+N94R+D111A+T114I+S216P+L227G+T231R+N233R+P256T、から選択された置換組を含んで成るリパーゼ。
【請求項１０】
薬剤として使用のためにプロテアーゼ又はアミラーゼと組合しての請求項１〜２及び５〜６のいずれか１項記載のリパーゼ。
【請求項１１】
薬剤として使用のためにプロテアーゼ及びアミラーゼと組合しての請求項１〜２及び５〜６のいずれか１項記載のリパーゼ。
【請求項１２】
（i）前記プロテアーゼが、
ａ）配列番号３のアミノ酸１〜274の配列を有するプロテアーゼ、
ｂ）配列番号４のアミノ酸１〜188の配列を有するプロテアーゼ、及び
ｃ）配列番号５のアミノ酸１〜188の配列を有するプロテアーゼから成る群から選択されたプロテアーゼに対して少なくとも70％の同一性を有し；
（ii）前記アミラーゼが、
ａ）配列番号６のアミノ酸１〜481の配列を有するアミラーゼ、
ｂ）配列番号７のアミノ酸１〜481の配列を有するアミラーゼ、及び
ｃ）配列番号８のアミノ酸１〜483の配列を有するアミラーゼから成る群から選択されたアミラーゼに対して少なくとも70％の同一性を有する請求項10又は11記載のプロテアーゼ及び/又はアミラーゼと組合してのリパーゼ。
【請求項１３】
消化障害、膵臓外分泌機能不全、膵炎、嚢胞性線維症、糖尿病Ｉ型及び/又は糖尿病II型の処理のための薬剤の製造のためへの請求項１〜２及び５〜６のいずれか１項記載のリパーゼ又はリパーゼ混合物の使用。
【請求項１４】
プロテアーゼ又はアミラーゼの使用をさらに含んで成る請求項13記載の使用。
【請求項１５】
プロテアーゼ及びアミラーゼの使用をさらに含んで成る請求項13記載の使用。
【請求項１６】
前記プロテアーゼ及び/又はアミラーゼが、請求項12に記載の通りである請求項14又は15記載の使用。
【請求項１７】
消化障害、膵臓外分泌機能不全、膵炎、嚢胞性線維症、糖尿病Ｉ型及び/又は糖尿病II型の処理への使用のためへの請求項１〜２及び５〜６のいずれか１項記載のリパーゼ。
【請求項１８】
プロテアーゼ又はアミラーゼと組合しての請求項17記載のリパーゼ。
【請求項１９】
プロテアーゼ及びアミラーゼと組合して請求項17記載のリパーゼ。
【請求項２０】
前記プロテアーゼ及び/又はアミラーゼが請求項12記載の通りである請求項18又は19記載のリパーゼ。
【請求項２１】
請求項１〜２及び５〜６のいずれか１項記載のリパーゼ又はリパーゼ混合物、及び少なくとも１つの医薬的に許容できる助剤材料を含んで成る医薬組成物。
【請求項２２】
プロテアーゼ又はアミラーゼをさらに含んで成る請求項21記載の組成物。
【請求項２３】
プロテアーゼ及びアミラーゼをさらに含んで成る請求項21記載の組成物。
【請求項２４】
前記プロテアーゼ及び/又はアミラーゼが請求項12記載の通りである請求項22又は23記載の組成物。
【請求項２５】
治療的有効量の請求項１〜２及び５〜６のいずれか１項記載のリパーゼ又はリパーゼ混合物を投与することによる、消化障害、膵臓外分泌機能不全、膵炎、嚢胞性線維症、糖尿病Ｉ型及び/又は糖尿病II型の処理方法。
【請求項２６】
治療有効量のプロテアーゼ又はアミラーゼを投与することをさらに含んで成る請求項25記載の方法。
【請求項２７】
治療有効量のプロテアーゼ及びアミラーゼを投与することをさらに含んで成る請求項25記載の方法。
【請求項２８】
前記プロテアーゼ及び/又はアミラーゼが請求項12記載の通りである請求項26又は27記載の方法。
【請求項２９】
（ａ）配列番号２のアミノ酸１〜269の配列に対して少なくとも50％の同一性を有し；そして
（ｂ）リパーゼ活性を有し；そして
（ｃ）次の置換：N26I, D27Q, D27R, D27Y, P29T, A30T, A30V, T32I, N33Q, N33T, N33Y, P42L, E43D, E43K, E43M, E43V, A49T, E56A, E56C, E56K, E56R, E56S, D57A, D57G, D57N, V60L, L69I, E87K,G91A, G91E, G91N, G91R, G91S, G91T, G91V, G91W, L93F, N94K, N94R, N94S, D96E, D96G, D96L, D96N, D96S, D96V, D96W, D96Y, L97M,L97Q, K98I, E99D, E99K, E99P, E99S, E99T, D111A, D111S, T114I, L147S, G163K, E210D, S216P, L227G, T231 R, N233R, D234K, E239V, Q249R, N251S, D254N, P256T, G263Q, L264A, I265T, G266D, T267A及びL269Nから選択された少なくとも１つの置換を含んで成り、ここで個々の位置は配列番号２のアミノ酸１〜269の位置に対応し；そして
（ｄ）但し、
（i）配列番号１のアミノ酸１〜269を有するリパーゼではなく、そして
（ii）(i)のリパーゼの変異体N33Qではない、親リパーゼの変異体である、薬剤として使用するためのリパーゼ。
【請求項３０】
（ａ）配列番号２のアミノ酸１〜269に対して少なくとも50％の同一性を有し；そして
（ｂ）リパーゼ活性を有し；そして
（ｃ）位置30、42、114及び/又は163の少なくとも１つの位置で少なくとも１つの置換を含んで成る（ここで、個々の位置は配列番号２のアミノ酸１〜269の位置に対応する）、親リパーゼの変異体である、薬剤として使用するためのリパーゼ。
【請求項３１】
（ａ）配列番号２のアミノ酸１〜269に対して少なくとも50％の同一性を有し；そして
（ｂ）リパーゼ活性を有し；そして
（ｃ）次の置換：A30T、A30V、P42L、T114I及びG163Kから選択された少なくとも１つの置換を含んで成る（ここで、個々の位置は配列番号２のアミノ酸１〜269の位置に対応する）、親リパーゼの変異体である、薬剤として使用するためのリパーゼ。
【請求項３２】
（ａ）配列番号２のアミノ酸１〜269に対して少なくとも50％の同一性を有し；そして
（ｂ）リパーゼ活性を有し；そして
（ｃ）次の変異体：
LV1232 G91A+D96W+E99K+G263Q+L264A+I265T+G266D+T267A+L269N;
LV1330 N33Q+D96S+T231R+N233R+Q249R;
LV1855 D27R+G91A+D111A+S216P+L227G+P256T;
LV1857 D27R+G91N+N94R+D111A+S216P+L227G+P256T;
LV1865 D27R+G91T+N94S+D111A+S216P+L227G+P256T;
LV1874 D27R+G91S+D111A+S216P+L227G+P256T;
LV1889 D27R+G91T+D96N+D111A+S216P+L227G+P256T;
LVA049 N33Q+G163K+T231R+N233R;
LVAR0002b T32I+G91V+T231R+N233R;
LVAR0003 K98I+T231R+N233R;
LVAROO11a G91A+T231R+N233R;
LVAR0013 G91V+T231R+N233R;
LVAR0014 N33Y+G91W+N94K+T231R+N233R;
LVAR0015 P42L+D57N+G91E+T231R+N233R;
LVAR0016 K98I+T231R+N233R;
LVAR0017 V60L+G91V+T231R+N233R;
LVAR0032 D57G+L93F+T231R+N233R;
LVAR0045 A49T+E56R+E87K+E99S+T231R+N233R;
LVAR0046 E99T+T114I+D254N+T231R+N233R;
LVAR0047 D27Y+E87K+D96L+E99P+T231R+N233R;
LVAR0048 E43K+E56S+E87K+T231R+N233R;
LVAR0050 E56S+E87K+D96L+E99D+T231R+N233R;
LVAR0051 E56A+D57A+T114I+T231R+N233R;
LVAR0052 G91E+T231R+N233R;
LVAR0053 E56K+D96G+D111A+T231R+N233R;
LVAR0054 E87K+D111S+T231R+N233R;
LVAR0055 E43V+G91R+T231R+N233R;
LVAR0056 E56S+E87K+T231R+N233R;
LVAR0057 E87K+G91E+T231R+N233R;
LVAR0058 D27Y+E87K+T231R+N233R;
LVAR0059 E43M+E87K+D96L+E99P+T231R+N233R;
LVAR0061 E56K+E87K+D111A+T231R+N233R;
LVAR0062 E87K+E99P+T231R+N233R;
LVAR0063 E87K+D96L+E99P+T231R+N233R;
LVAR0064 E56C+E87K+T231R+N233R;
LVAR0065 E56R+E87K+D96L+T231R+N233R;
LVAR0066 E43D+E56A+D57A+E87K+D111A+T231R+N233R;
LVAR0067 E56K+E87K+D96L+E99P+T231R+N233R;
LVAR0068 E87K+L147S+T231R+N233R;
LVAR0069 D27Y+E87K+D96L+E99P+T231R+N233R;
LVAR0070 E43D+E87K+D96L+E99P+E239V+T231R+N233R;
LVAR0071 E43K+E56A+E87K+D234K+T231R+N233R;
LVAR0072 D96V+D111A+T231R+N233R; 及び
LVAR0078 N33T+E43V+E56K+D96G+T231R+N233R；
から選択される、親リパーゼの変異体である、薬剤として使用するためのリパーゼ。
【請求項１】
（ａ）配列番号２のアミノ酸１〜269の配列に対して少なくとも50％の同一性を有し；
（ｂ）リパーゼ活性を有し；そして
（ｃ）配列番号2のアミノ酸１〜269の配列に比較して、置換N33Q、T231R及びN233R、並びに下記：
E1*,D,N；Q4H,P,R；D5E；N8L,Q；Q9H；F10L; N11C,D,H,L,P,Q,R,S; G23E; N26A,H,I；
D27I,N,Q,R,S,V; P29T; A30T,V; T37K,M; G38A,D,F,H,I,K,L,M,N,P,Q1S,T,W,Y; N39H,S; E43K;K46M; A49T; L52I,R; E56K,Q,R,S; D57G,N; V60E,S; G61R; V63R; A68V; L69I; N71I,S;N73Q,Y; I76T; R84E; I86F,L; E87A,H,K,R; I90L,V; G91A,C,E,F,K,L,M,N,S,T,V,W,Y; L93*,F;N94*K,Q,R,S;F95*;D96*,E,G,N,R,S,W,Y;L97M,Q;K98I,T;E99D;N101Q;D102E,G,Y;
R108M;G109A;D111A,E,N,S;G112A;T114I;S115L;W117C,D,E,F,G,H,I,K,L,P,S,T,V,Y;D122E,N;Q126L;V128A;D130H;H135D;P136H;Y138F;V141E,L;A150V;V154F,I,L;A155V;G156R;G161A,E;N162G,S,T;G163A,C,D,E,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y;D167E;V168M;V176A,D,F,G,H,I,K,M,N,Q,T,W; G177A; R179T; L185M; G190C,D; N200Q,S; R205I;L206F; E210D,R,V,Y; S216P; E219D; G225P; T226N; L227F,G; P229R; E239D; G240L; D242E; T244S; G246A;Q249R;N251Q,S;D254A,G,I,K,L,M,N,R,Q,S,Y;I255A,F;P256A,F,G,H,I,L,M,N,Q,S,T,V,W,Y; 及び L269F,Hから選択された少なくとも１つの追加の置換を含んで成る、薬剤として使用するためのリパーゼ。
【請求項２】
次の置換：N33Q+T231R+N233Rを有する、配列番号２の配列を有する対照リパーゼに比較して、インビトロで改良された消化性能を有する請求項１記載のリパーゼ。
【請求項３】
ａ）対照リパーゼを選択し；
ｂ）それぞれ、100部/体積の食餌と、20部/体積のペプシン及び30部/体積のリパーゼ又は対照リパーゼとを混合し；
ｃ）i)０又はii)10部/体積の緩衝液（0.8MのMES、0.8Mの酢酸ナトリウム、0.8Mのイミダゾール、pH7.0）を添加し、ここで段階i)はpH3の胃段階として言及され得、そして段階ii)はpH5の胃段階として言及され得；
ｄ）振盪下で37℃で１時間インキュベートし；
ｅ）20部/体積の胆汁塩、及びi)25又はii)15部/体積の緩衝液（0.8MのMES、0.8Mの酢酸ナトリウム、0.8Mのイミダゾール、pH7.0）を添加し、ここで段階i)はpH3の胃段階として言及され得、そして段階ii)はpH5の胃段階として言及され得；
ｆ）振盪下で37℃で２時間インキュベートし；
ｇ）1Mのリン酸中、50部/体積の10％Triton-X100を添加し；
ｈ）遊離脂肪酸の体積を決定し；
i)下記等式：
FFA＝FFAmax*[E]/（[E]＋K）
[式中、FFAは開放される遊離脂肪酸の量であり、FFAmaxは、リパーゼ食餌から遊離することができる遊離脂肪酸の最大量であり、「E」はリパーゼ濃度であり、そしてKはFFAmaxの半分を遊離するリパーゼ濃度である]に、用量応答曲線を適合し；
ｊ）次の通りに改良因子（IF）：
IF＝K（対照）/K(リパーゼ)
[ここでK（対照）は、FFAmaxの半分を遊離する対照リパーゼの濃度であり、そしてK（リパーゼ）は、FFAmaxの半分を遊離するリパーゼ濃度である]を計算する段階を含んで成る、対照リパーゼに比較して、リパーゼのインビトロ消化性能を決定するための方法。
【請求項４】
ａ）i)対照リパーゼを選択し；
ii)それぞれ、５部/体積のリパーゼ又は対照リパーゼと、５部/体積の
ａ）0.001％のTriton−X100及び10mMのNaClを含む希釈剤、又は
ｂ）150μg/mlのペプシン、4mMのCaCl2、0.01％のTriton-X100及び50mMのシトレート、pH3.0を含むペプシン処理溶液（ここでa）は未処理のサンプルとして言及され、そしてｂ）はペプシン処理されたサンプルとして言及される）とを混合し；
iii)段階ii)のサンプルを、20℃で３時間インキュベートし；
iv)段階iii)の個々のサンプルに、1mMのPNP−パルミテート、1.2％のTriton-X100、4mMのCaCl2、100mMのTRIS, pH8.0を含む、55部/体積の基質、及び
ａ）5部/体積のペプシン処理溶液、又は
ｂ）5部/体積の希釈剤（ここでａ）は未処理のサンプルを言及し、そしてｂ）はペプシン処理されたサンプルを言及する）を添加し；
v）iv)サンプルのOD405を一定間隔で読み取ることにより基質の分解を追跡し；
vi)直線範囲に落ちるv）からのデータを集め、そしてそれぞれ、ペプシン処理されたサンプル及び未処理のサンプルについて、mOD（ミリOD）/時でのリパーゼ活性を計算し；
vii)ペプシン処理されたサンプルのリパーゼ活性を、未処理のサンプルのリパーゼ活性により、それらが段階vi)に起因する場合、割算し、そしてその結果に100を掛算することにより％残留リパーゼ活性（％RA）を計算し；そして所望には、
viii)リパーゼの％RAと、対照リパーゼの％RAとを比較することにより、ペプシンの存在下でpH3での安定性を決定し、そして/又は
ｂ）i)対照サンプルを選択し；
ii)それぞれ、10部/体積のリパーゼ又は対照リパーゼと、23部/体積のａ）水、又はｂ）20mMの胆汁塩とを混合し、ここでａ）は未処理のサンプルとして言及され、そしてｂ）は胆汁塩サンプルとして言及され；
iii)ii)の個々のサンプルに、25mMのスクシネート中、1mMのPNPオレエート、2mMのCaCl2、1.2％のTriton−X100、pH5.0を含む、200部/体積の基質を転化し；
iv)段階iii)の直後、個々のサンプルから、60部/体積の前記得られる混合物を除き、そしてその15部/体積を、４つの別々の区画中に４度、移し；
v）１，２，３及び４時間後、60部/体積の100mMのTRIS（pH8.0）を、iv)の４つの区画のそれぞれの区画に添加し、すぐにOD405を読み取り、そして１，２，３及び４時間の読み取りの線状範囲に基づいて、mOD/時での活性を計算し；
vi)それぞれ、リパーゼ及び対照リパーゼの胆汁塩安定性比率に達するよう、リパーゼ及び対照リパーゼについて、胆汁塩サンプルの段階v)で得られる活性を、段階v）でまた得られるような未処理のサンプルの活性により割算し；そして
vii)リパーゼの胆汁塩安定性比率を、対照リパーゼの胆汁塩を安定性比率により割算する（得られる比率はリパーゼの改良因子として定義され得る）ことにより、胆汁塩の存在下でpH5での活性を定義する段階を含んで成る、対照リパーゼに比較して、リパーゼのインビトロでの消化性能を決定するための方法。
【請求項５】
前記インビトロでの消化性能が請求項３又は４のいずれか１項記載の方法を用いて決定される請求項２記載のリパーゼ。
【請求項６】
下記：
D27R+N33Q+G91A+D96E+L97Q+D111A+T231R+N233R+P256T;
N33Q+E210D+T231R+N233R;
N33Q+D111A+T231R+N233R;
N33Q+G91T+T231R+N233R;
N33Q+E219D+T231R+N233R;
N33Q+W117L+T231R+N233R;
D27Q+N33Q+T231R+N233R;
N33Q+G91T+T231R+N233R;
D27S+N33Q+G91A+D96E+L97Q+D111A+S216P+T231R+N233R+P256T;
D27R+N33Q+G91N+N94R+D111A+T231R+N233R+P256T;
D27R+N33Q+G91T+N94S+D111A+S216P+L227G+T231R+N233R+P256T;
Q4R+N33Q+T231R+N233R;
N33Q+T231R+N233R+Q249R;
N33Q+D96W+T231R+N233R;
D27V+N33Q+V60S+D96W+T231R+N233R+Q249R;
D27V+N33Q+V60S+T231R+N233R+Q249R;
Q9H+N33Q+D102E+T231R+N233R;
N33Q+D111E+T231R+N233R;
N33Q+D122E+T231R+N233R;
D27R+N33Q+G91N+N94R+D111A+S216P+L227G+T231R+N233R+P256T;
N33Q+D167E+T231R+N233R;
N33Q+G91N+T231R+N233R;
N33Q+T231R+N233R+P256T;
D27R+N33Q+G91A+L93*+N94*+F95*+D96*+D111A+T231R+N233R+P256T;
N11R+N33Q+T231R+N233R;
N33Q+N39H+T231R+N233R;
N33Q+P229R+T231R+N233R;
D27R+N33Q+G91N+N94R+D111A+G163K+S216P+L227G+T231R+N233R+P256T;
N33Q+G91T+G163K+T231R+N233R;
D27R+N33Q+G91A+D96E+L97Q+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+G91A+D96E+L97Q+D111A+S216P+T231R+N233R+P256T;
N33Q+E87A+T231R+N233R;
N33Q+E56Q+T231R+N233R;
N33Q+E210V+T231R+N233R;
N33Q+E56K+T231R+N233R;
N33Q+T231R+N233R+D254G;
N33Q+D96S+T231R+N233R;
N33Q+D122N+T231R+N233R;
N26A+N33Q+T231R+N233R;
N33Q+N162T+T231R+N233R;
N33Q+A150V+N162G+T231R+N233R;
N33Q+I90L+G163L+T231R+N233R;
N33Q+T231R+N233R+G240L;
D27R+N33Q+G91A+D96E+D111A+T231R+N233R+D254G+P256T;
D27R+N33Q+G91A+N94S+D111A+T231R+N233R+P256T;
N33Q+N200S+T231R+N233R;
N33Q+N39S+T231R+N233R;
N33Q+E210R+T231R+N233R;
N33Q+N39H+T231R+N233R+D254R;
N33Q+T231R+N233R+D254R;
N33Q+N94R+T231R+N233R;
N33Q+D96R+T231R+N233R;
D27N+N33Q+T231R+N233R;
D27N+N33Q+E56R+T231R+N233R;
N33Q+L227F+T231R+N233R;
N33Q+N73Y+G225P+T231R+N233R;
N33Q+G225P+T231R+N233R;
N33Q+T231R+N233R+D254S;
N33Q+D96G+T231R+N233R;
N33Q+D96N+T231R+N233R+D254S;
N33Q+T231R+N233R+D254G;
N33Q+D130H+T231R+N233R;
N33Q+E87A+T231R+N233R;
N33Q+T231R+N233R+E239D;
N33Q+D111A+T231R+N233R+D254G;
N33Q+E210V+T231R+N233R+D254S;
N11R+N33Q+E210V+T231R+N233R+D254S;
N33Q+G91T+G163K+T231R+N233R+D254G;
N33Q+G91T+G163K+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+T231R+N233R+D254S;
Q4R+D27R+N33Q+G91T+N94S+D111A+S216P+L227G+T231R+N233R+P256T;
N33Q+G91T+N94S+D111A+V176I+T231R+N233R;
Q4R+D27R+N33Q+G91T+N94S+D111A+E210D+S216P+L227G+T231R+N233R+P256T;
Q4R+D27Q+N33Q+G91T+N94S+D111A+S216P+L227G+T231R+N233R+P256T;
N33Q+G91T+N94S+D111A+T231R+N233R+P256T;
N33Q+G177A+T231R+N233R;
N33Q+T231R+N233R+G246A;
D27N+N33Q+G91T+G163K+T231R+N233R+D254S;
D27Q+N33Q+G91T+G163K+E219D+T231R+N233R;
N33Q+G91T+E219D+T231R+N233R;
K98I+T231R+N233R+N251S;
N33Q+G163R+T231R+N233R;
N33Q+G163N+T231R+N233R;
N33Q+G163C+T231R+N233R;
N33Q+G163Q+T231R+N233R;
N33Q+G163E+T231R+N233R;
N33Q+G163H+T231R+N233R;
N33Q+G163I+T231R+N233R;
N33Q+G163P+T231R+N233R;
N33Q+G163D+T231R+N233R;
N33Q+G91K+T231R+N233R;
N33Q+G91M+T231R+N233R;
N33Q+G91F+T231R+N233R;
N33Q+G91S+T231R+N233R;
N33Q+G91W+T231R+N233R;
N33Q+G91Y+T231R+N233R;
N33Q+G163T+T231R+N233R;
N33Q+G163W+T231R+N233R;
N33Q+G163Y+T231R+N233R;
N33Q+G163V+T231R+N233R;
N33Q+G91C+T231R+N233R;
N33Q+G91Y+Q126L+T231R+N233R;
N33Q+G91M+G161E+T231R+N233R;
N33Q+V128A+T231R+N233R;
N33Q+G163V+L185M+T231R+N233R;
N33Q+G38A+T231R+N233R;
N33Q+G163A+T231R+N233R;
N33Q+G91T+N94S+D111A+T231R+N233R;
N33Q+G38A+G163A+T231R+N233R;
N33Q+G163M+T231R+N233R;
N33Q+G91V+T231R+N233R;
N33Q+D111A+T231R+N233R+Q249R;
N33Q+D111A+T231R+N233R+D254S;
D27R+N33Q+G91A+D96E+L97Q+D111A+T231R+N233R+D254S+P256T;
D27R+N33Q+G91A+D96E+L97Q+D111A+T231R+N233R+D254G+P256T;
N33Q+G91T+N94R+T231R+N233R+D254S;
N33Q+G91T+N94R+D111A+W117L+T231R+N233R;
N33Q+W117L+T231R+N233R+D254S;
N33Q+T231R+N233R+P256T;
N33Q+T231R+N233R+D242E;
N33Q+E87R+T231R+N233R;
N33Q+E56R+T231R+N233R;
N33Q+N162G+T231R+N233R;
N33Q+G91L+T231R+N233R;
N33Q+E87H+T231R+N233R;
N33Q+D96N+T231R+N233R+Q249R;
N33Q+G91T+N94R+T231R+N233R+D254S;
N33Q+L227F+T231R+N233R+D254S;
D27R+N33Q+G91T+D96E+L97Q+D111A+T231R+N233R+D254S+P256T;
N33Q+G163A+T231R+N233R;
D27R+N33Q+G91T+D96E+D111A+T231R+N233R+D254S+P256T;
N33Q+G91T+N94R+T231R+N233R;
N33Q+T231R+N233R+D254A;
N33Q+T231R+N233R+D254N;
N33Q+T231R+N233R+D254Q;
N33Q+T231R+N233R+D254I;
N33Q+T231R+N233R+D254L;
N33Q+T231R+N233R+D254K;
N33Q+T231R+N233R+D254M;
N33Q+S216P+L227G+T231R+N233R+Q249R;
D27V+N33Q+V60S+G91T+D96W+T231R+N233R+Q249R;
N33Q+D96N+L227G+T231R+N233R+Q249R;
D27R+N33Q+L227G+T231R+N233R;
D27R+N33Q+L227G+T231R+N233R+Q249R;
N33Q+E219D+L227G+T231R+N233R+Q249R;
D27Q+N33Q+L227G+T231R+N233R+Q249R;
N33Q+W117L+L227G+T231R+N233R+Q249R;
D5E+N33Q+W117L+L227G+T231R+N233R+Q249R;
D27Q+N33Q+E219D+L227G+T231R+N233R+Q249R;
N33Q+D96E+E219D+L227G+T231R+N233R+Q249R;
D27R+N33Q+E56K+G91N+N94R+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+E56Q+D57N+G91N+N94R+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+S216P+L227G+T231R+N233R+D254S+P256T;
D27R+N33Q+E56S+G91N+N94R+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+G91N+N94R+D111A+S216P+L227G+T231R+N233R+D254S+P256T;
D27R+N33Q+G91N+N94R+D111A+S216P+L227G+T231R+N233R+D254S+P256T;
D27R+N33Q+G91N+N94R+D111S+A155V+S216P+L227G+T231R+N233R+D254S+P256T;
D27R+N33Q+G91N+N94R+D111S+S216P+L227G+T231R+N233R+D254S+P256T;
N33Q+D111A+T231R+N233R+D254S;
N33Q+D111A+W117L+T231R+N233R+D254S;
N33Q+T231R+N233R+P256A;
N33Q+T231R+N233R+P256N;
N33Q+T231R+N233R+P256G;
N33Q+T231R+N233R+P256H;
N33Q+T231R+N233R+P256L;
N33Q+T231R+N233R+P256M;
N33Q+T231R+N233R+P256S;
N33Q+T231R+N233R+P256W;
N33Q+T231R+N233R+P256Y;
N33Q+T231R+N233R+P256F;
N33Q+T231R+N233R+P256V;
N33Q+G91M+G163W+T231R+N233R;
N33Q+G91M+G163T+T231R+N233R;
N33Q+G91M+G163D+T231R+N233R;
N33Q+G91K+G163W+T231R+N233R;
N33Q+G91T+G163W+T231R+N233R;
N33Q+V176N+T231R+N233R;
N33Q+V176D+T231R+N233R;
N33Q+W117F+T231R+N233R;
N33Q+G91T+N94S+D111A+V176I+T231R+N233R+D254S;
N33Q+V176I+T231R+N233R;
N33Q+D111N+T231R+N233R;
N33Q+D111N+G225P+T231R+N233R;
N33Q+D111N+S216P+T231R+N233R;
D27R+N33Q+G91T+N94R+D111A+S216P+L227G+T231R+N233R;
N33Q+G91M+G163P+T231R+N233R;
N33Q+G91T+G163A+T231R+N233R;
N33Q+W117D+T231R+N233R;
N33Q+W117H+T231R+N233R;
N33Q+W117C+T231R+N233R;
N33Q+W117K+T231R+N233R;
N33Q+W117V+T231R+N233R;
N11S+N33Q+T231R+N233R;
N33Q+W117E+V176K+T231R+N233R;
N33Q+W117G+T231R+N233R;
N33Q+W117P+T231R+N233R;
N33Q+W117S+T231R+N233R;
N33Q+ W117T+T231R+N233R;
N33Q+W117I+T231R+N233R;
D27R+N33Q+ L227G +T231R+N233R+Q249R+D254S;
N33Q+S115L+T231R+N233R;
N33Q+G38A+G91T+G163K+T231R+N233R+D254S;
N33Q+V176M+T231R+N233R;
N33Q+V176H+T231R+N233R;
N33Q+V176A+T231R+N233R;
D27V+N33Q+L227F+T231R+N233R+Q249R;
N33Q+W117Y+T231R+N233R;
N33Q+W117Y+V176D+T231R+N233R;
D27V+N33Q+G91A+N94R+D111A+G163K+L227F+T231R+N233R+Q249R;
D27V+N33Q+G91A+N94R+D111A+G163K+L227F+T231R+N233R+Q249R+D254S;
D27R+N33Q+P136H+L227G+T231R+N233R+Q249R+D254S;
N11R+N33Q+T231R+N233R+T244S;
N33Q+G91T+D96N+D111A+V176I+T231R+N233R+D254S;
N33Q+G91T+N94S+D111A+V176I+T231R+N233R+D254S;
N33Q+G161A+T231R+N233R;
N33Q+G38I+G177A+T231R+N233R;
N33Q+N101Q+T231R+N233R;
N33Q+N94Q+T231R+N233R;
N33Q+G161A+T231R+N233R;
N11Q+N33Q+T231R+N233R;
N8Q+N33Q+T231R+N233R;
N33Q+T231R+N233R+N251Q;
N33Q+N200Q+T231R+N233R;
N33Q+G177A+T231R+N233R;
N33Q+N73Q+T231R+N233R;
N33Q+I86L+T231R+N233R;
N33Q+K98I+G163K+T231R+N233R;
D27R+N33Q+G91T+D96E+D111A+G163K+T231R+N233R+D254S+P256T;
D27R+N33Q+G91T+D96E+D111A+G163A+T231R+N233R+D254S+P256T;
D27R+N33Q+S216P+L227G+T231R+N233R+Q249R;
N33Q+K98I+G163K+N200Q+T231R+N233R+N251S;
N33Q+G38S+G163K+T231R+N233R;
D27R+N33Q+G38A+G91T+D96E+D111A+T231R+N233R+D254S+P256T;
N33Q+G38Y+T231R+N233R;
D27R+N33Q+G91T+N94R+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+G91T+N94R+D111A+S216P+L227G+T231R+N233R+P256T;
N33Q+G38N+N73Q+T231R+N233R;
N33Q+G38D+R84E+T231R+N233R;
N33Q+G38Q+T231R+N233R;
N33Q+G38I+T231R+N233R;
N33Q+G38K+T231R+N233R;
N33Q+G38F+T231R+N233R;
N33Q+G38H+N200Q+T231R+N233R+N251S;
N33Q+G38L+T231R+N233R;
N33Q+G38M+T231R+N233R;
N33Q+G38F+T231R+N233R;
N33Q+G38P+T231R+N233R;
N33Q+G38T+T231R+N233R;
N11R+N33Q+G91T+W117I+G163K+T231R+N233R+D254S;
D27R+N33Q+G38A+G91T+D96E+D111A+G163K+T231R+N233R+D254S+P256T;
N11R+N33Q+G91T+W117I+G163K+T231R+N233R+D254S;
D27R+N33Q+G38A+G91T+D96E+D111A+G163A+T231R+N233R+D254S+P256T;
D27R+N33Q+V176Q+L227G+T231R+N233R+Q249R+D254S;
N33Q+W117I+V176Q+T231R+N233R+P256A;
N33Q+G38A+G163A+T231R+N233R+P256A;
N33Q+W117I+V176Q+T231R+N233R;
N33Q+G177A+T231R+N233R+G246A;
E1N+N33Q+T231R+N233R;
N33Q+G38H+T231R+N233R;
N33Q+G91A+N94K+D111A+G163K+L227F+T231R+N233R+Q249R+D254S;
N11R+N33Q+G91T+G163K+V176Q+T231R+N233R+D254S;
N33Q+K98I+T231R+N233R;
D27R+N33Q+W117I+V176Q+L227G+T231R+N233R+Q249R+D254S;
N11R+N33Q+G38A+G91T+G163K+T231R+N233R+D254S;
N33Q+G163W+T231R+N233R;
N33Q+G38A+G163A+T231R+N233R;
D27R+N33Q+G91T+D96E+L97Q+D111A+T231R+N233R+D254S+P256T;
N33Q+T231R+N233R+D254Q;
N11R+N33Q+G91T+S115L+G163K+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+V176W+T231R+N233R+D254S;
N33Q+G163D+T231R+N233R;
N33Q+G163P+T231R+N233R;
E1D+N33Q+G91T+N94R+D111A+W117L+T231R+N233R+D254S;
N33Q+G91T+N94R+D111A+W117L+V176W+T231R+N233R;
Q4P+D27R+N33Q+G91N+N94R+D111A+L206F+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+T37K+N711+G91N+N94R+K98I+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+E43K+K46M+I90V+G91N+N94R+D111A+T114I+S216P+L227G+T231R+N233R+P256T;
N33Q+W117S+T231R+N233R;
N33Q+G61R+V63R+G156R+V176W+T231R+N233R+P256I;
N33Q+D96N+G156R+V176W+T231R+N233R;
N33Q+G156R+V176W+T231R+N233R+Q249R;
N33Q+G91T+N94S+D111A+G163T+V176W+T231R+N233R;
N33Q+G91T+N94S+D111A+S115L+G163T+V176I+T231R+N233R;
N11R+D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+G163T+S216P+L227G+T231R+N233R+D254S+P256T;
D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+G163T+S216P+L227G+T231R+N233R+D254S+P256T;
N11R+D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+S216P+L227G+T231R+N233R+D254S+P256T;
D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+S216P+L227G+T231R+N233R+D242E+D254S+P256T;
D27R+N33Q+G38A+E56Q+D57N+G91N+N94R+D111S+S216P+L227G+T231R+N233R+D254S+P256T;
Q4R+D27Q+N33Q+G91T+N94S+E99D+D111A+E210D+S216P+L227G+T231R+N233R+P256L;
N33Q+G38A+G91T+G163A+T231R+N233R+D254S;
N33Q+G38A+G163A+T231R+N233R+D254I;
N11R+N33Q+I90L+G163L+T231R+N233R;
N11R+N33Q+I90L+G163L+T231R+N233R+D254S;
N11R+N33Q+E56Q+G91T+G163K+V176Q+T231R+N233R+D254S;
N11R+D27R+N33Q+G91T+D96E+D111A+G163K+T231R+N233R+D254S+P256T;
N11R+N33Q+G38A+G91T+G112A+G163A+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+E210D+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+T231R+N233R+D254I;
N11R+N33Q+G91T+G163K+V176T+T231R+N233R+D254S;
N11R+N33Q+G91T+G163P+T231R+N233R+D254S;
N11R+N33Q+G91M+G163T+T231R+N233R+D254S;
N11R+N33Q+G38A+G91T+G163K+V176D+T231R+N233R+D254S;
N33Q+E56Q+G156R+V176W+T231R+N233R;
E1D+N33Q+G38A+G91T+N94R+D111A+W117L+V176W+T231R+N233R;
N33Q+G163K+G177A+T231R+N233R+G246A;
N11R+N33Q+E56Q+G91T+G163K+T231R+N233R+D254S;
N11R+N33Q+I90L+G163K+T231R+N233R+D254S;
D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+S216P+L227G+T231R+N233R+Q249R+D254S+P256T;
D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+S216P+E219D+L227G+T231R+N233R+D254S+P256T;
N11R+N33Q+I90L+G91T+N94S+D96E+G163K+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+V176I+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+V176Q+T231R+N233R+D254S;
N11R+N33Q+G91T+G163A+V176T+T231R+N233R+D254S;
N11R+N33Q+G91T+G163L+V176I+T231R+N233R+D254S;
N11R+N33Q+G91T+G163L+V176T+T231R+N233R+D254S;
N11R+N33Q+G91T+G163L+T231R+N233R+D254S;
N11R+N33Q+G91T+G163P+T231R+N233R+D254S;
N11R+N33Q+G91T+G163P+V176I+T231R+N233R+D254S;
N11R+N33Q+G91T+G163L+T231R+N233R+D254S+P256N;
D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+G163T+S216P+L227G+T231R+N233R+Q249R+D254S+P256T;
Q4R+D27Q+N33Q+G91T+N94S+E99D+D111A+G163A+E210V+S216P+L227G+T231R+N233R+P256L;
Q4R+D27Q+N33Q+G91T+N94S+E99D+D111A+V176I+E210V+S216P+L227G+T231R+N233R+P256L;
N33Q+E210Y+T231R+N233R+D254Y+I255F;
N33Q+L93F+D102Y+T231R+N233R;
D27R+N33Q+L227G+T231R+N233R+Q249R+D254S;
N11S+N33Q+T231R+N233R;
N11R+N33Q+T231R+N233R;
N33Q+G38A+G91T+G163K+T231R+N233R+D254S;
N33Q+W117Y+V176T+T231R+N233R;
N8L+N11R+N33Q+G91T+G163K+T231R+N233R+D254S;
E1N+N33Q+G38A+G91T+G163P+V176F+T231R+N233R;
N11R+N33Q+G38A+G91T+G163P+V176G+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+T231R+N233R+D254A+P256F;
N11R+N33Q+G91T+G163K+T231R+N233R+P256F;
N11R+N33Q+G91T+G163K+T231R+N233R+D254S+P256F;
N11R+N33Q+G38A+G91T+G156R+G163K+V176T+T231R+N233R+D254S;
N33Q+G91K+D96S+G163T+T231R+N233R+Q249R;
N11R+N33Q+G91T+G163N+T231R+N233R+D254S;
N11R+N33Q+G91T+G163T+T231R+N233R+D254S;
N11R+N33Q+G91T+G163W+T231R+N233R+D254S;
N11R+N33Q+G91K+G163K+T231R+N233R+D254S;
N11R+G23E+N33Q+G91T+G163K+T231R+N233R+D254S;
N11R+N33Q+G91T+V141E+G163K+T231R+N233R+D254S;
N11R+N33Q+L52R+G91T+G163K+T231R+N233R+D254S;
N11R+N33Q+G91T+V141L+G163K+T231R+N233R+D254S;
N11R+N33Q+T37K+G91T+G163K+T231R+N233R+D254S;
N11R+N33Q+A68V+G91T+G163K+T231R+N233R+D254S;
N11R+N33Q+G91T+G163A+V176I+T231R+N233R+D254S;
N11R+N33Q+T37M+G91T+G163P+V176T+T231R+N233R+D254S;
N11R+N33Q+G91T+G163L+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+T231R+N233R+D254S+P256I;
N33Q+G38S+G156R+G163K+V176W+T231R+N233R;
N11R+D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+G163K+S216P+L227G+T231R+N233R+D254S+P256T;
N11R+N33Q+G38A+G91T+G163P+V176G+T231R+N233R+D254S;
N11R+N33Q+G38A+G91T+G163Q+V176G+T231R+N233R+D254S;
N11R+N33Q+G38A+G91T+G163T+V176G+T231R+N233R+D254S;
N11R+N33Q+G38A+G91T+N94R+G163P+V176G+T231R+N233R+D254S;
E1*+N11R+N33Q+G38A+G91N+N94R+G163P+V176G+T231R+N233R+D254S;
E1N+N11R+N33Q+G38A+G91T+G163P+V176F+T231R+N233R;
E1N+F10L+N11R+N33Q+G38A+G91T+G163P+V176F+T231R+N233R;
E1N+N33Q+G38A+G91T+G163P+V176F+T231R+N233R+D254S;
E1N+N33Q+G38A+G91T+D111A+G163P+V176F+T231R+N233R;
E1N+N33Q+G38A+G91T+G163P+V176F+L227F+T231R+N233R;
E1N+N11R+N33Q+G38A+G91T+D111A+G163P+V176F+T231R+N233R;
E1N+N33Q+G38A+G91T+G163P+V176F+L227F+T231R+N233R+D254S;
E1N+N33Q+G38A+G91T+G163P+V176F+T231R+N233R+D254S+I255A+P256Q;
E1N+N11R+N33Q+G38A+G91T+D111A+G163P+V176F+T231R+N233R+D254S;
N33Q+G156R+V176W+T231R+N233R+P256I;
N33Q+G91T+N94S+D111A+G156R+G163T+V176W+T231R+N233R;
N33Q+G91T+N94S+D111A+G156R+G163T+V176I+T231R+N233R;
N11R+N33Q+G38A+G91T+D102G+S115L+G163K+T231R+N233R+D254S+P256T;
N11R+N33Q+G38A+G91T+S115L+G163K+T231R+N233R+D254S+P256T;
E1N+N11R+N33Q+G91T+G163A+T231R+N233R+G246A+D254S;
N11R+D27R+N33Q+D57G+G91T+D96E+D111A+G163K+T231R+N233R+D254S+P256T;
N33Q+D96N+G156R+V176W+T231R+N233R+Q249R;
N33Q+I86F+L93F+D102Y+E210Y+L227F+T231R+N233R+D254Y+I255F+L269F;
N33Q+I86F+L93F+D102Y+E210Y+L227F+T231R+N233R+D254Y+I255F;
N11C+N33Q+G91T+G163K+T231R+N233R+D254S;
N11L+N33Q+G91T+G163K+T231R+N233R+D254S;
N11H+N33Q+G91T+G163K+T231R+N233R+D254S;
N11D+N33Q+G91T+G163K+T231R+N233R+D254S;
N11R+N33Q+G91T+D96W+G163K+T231R+N233R+D254S;
D27R+N33Q+G91T+D96E+L97Q+D111A+G163K+T231R+N233R+D254S+P256T;
N11P+N33Q+G91T+G163K+T231R+N233R+D254S;
Q4R+D27N+N33Q+G38A+G91T+N94S+E99D+D111A+V176I+E210V+S216P+L227G+T231R+N233R+P256L;
N11R+N33Q+E56Q+G163K+T231R+N233R+D254S;
N11R+N33Q+G91T+G163A+T231R+N233R+D254S;
N11R+N33Q+G91T+G163P+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+L227G+P229R+T231R+N233R+D254S;
N33Q+E87K+T231R+N233R;
N33Q+N94K+T231R+N233R;
N33Q+D96Y+T231R+N233R;
N33Q+K98I+T231R+N233R;
A30V+N33Q+K98I+T231R+N233R;
N33Q+E87K+D96E+T231R+N233R;
N26I+N33Q+T231R+N233R;
A30T+N33Q+T231R+N233R;
N33Q+G91V+T231R+N233R;
N33Q+G91A+T231R+N233R;
N33Q+G91V+L97M+T231R+N233R;
N33Q+K98I+T231R+N233R;
N33Q+L69I+G91E+T231R+N233R;
P29T+N33Q+T231R+N233R;
N33Q+G91V+T231R+N233R;
N33Q+K98I+T231R+N233R;
N33Q+G91E+T231R+N233R;
N33Q+N94K+T231R+N233R;
D27R+N33Q+G91N+N94R+K98I+D111A+N162S+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+T37K+N711+G91N+N94R+K98I+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+N39S+G91N+N94R+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+I76T+G91N+N94R+R108M+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+L52I+V60E+G91N+N94R+D111A+T114I+V168M+E210D+S216P+L227G+T231R+N233R+P256T;
Q4P+D27R+N33Q+G91N+N94R+D111A+R205I+L206F+S216P+L227G+T231R+N233R+P256T;
Q4H+D27R+N33Q+G91N+N94R+D111A+V154L+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+G91N+N94R+D111A+V154I+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+N71S+G91N+N94R+D111A+H135D+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+G91N+N94R+K98I+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+G91N+N94R+L97M+D111A+S216P+T226N+L227G+T231R+N233R+P256T+L269H;
D27R+N33Q+G91N+N94R+D111A+T114I+R179T+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+G91N+N94R+D111A+S216P+L227G+T231R+N233R；
G23E+D27R+N33Q+L52R+G91N+N94R+D111A+T114I+V141E+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+E43K+K46M+I90V+G91N+N94R+D111A+T114I+S216P+L227G+T231R+N233R+P256T;
D27R+A30V+N33Q+G91N+N94R+G109A+D111A+G190D+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+A49T+G91N+N94R+D111A+Y138F+G163R+S216P+L227G+T231R+N233R+P256T;
N26H+D27R+N33Q+G91N+N94R+D111A+V154F+G190C+S216P+L227G+T231R+N233R+P256T;
N33Q+G91T+D96E+K98T+T114I+G163S+E210V+T231R+N233R+D254K+P256A;
N33Q+G91T+D96E+K98T+T114I+T231R+N233R+G163S;
N33Q+G91T+D96E+K98T+T114I+G163K+E210D+T231R+N233R;
N33Q+G91T+T114I+G163K+E210D+T231R+N233R+D254G+P256A;
D27R+N33Q+G91T+T114I+G163W+E210D+T231R+N233R;
D27N+N33Q+G91T+T114I+G163S+E210D+T231R+N233R+P256T;
N33Q+G91T+T114I+G163K+E210D+T231R+N233R;
N33Q+G38W+G91T+T114I+G163K+E210V+T231R+N233R;
N33Q+G38W+G91T+T114I+G163K+E210D+T231R+N233R+P256T;
D27I+N33Q+G91T+D96E+K98T+T114I+G163K+E210D+T231R+N233R+P256T;
N33Q+G91T+T114I+E210V+T231R+N233R+D254K+P256A;
N33Q+G91A+N94K+D111A+G163K+L227F+T231R+N233R+Q249R;
G23E+D27R+N33Q+L52R+G91N+N94R+D111A+T114I+V141E+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+E43K+K46M+I90V+G91N+N94R+D111A+T114I+S216P+L227G+T231R+N233R+P256T;
N33Q+G91T+K98I+T114I+G163K+T231R+N233R+D254S;
N33Q+G91T+K98I+G163K+T231R+N233R+D254S+P256L;
N33Q+G91T+T114I+G163K+T231R+N233R+D254S+P256L;
G23E+D27R+N33Q+L52R+G91N+N94R+D111A+T114I+V141E+S216P+L227G+T231R+N233R+P256T; 及び
D27R+N33Q+E43K+K46M+I90V+G91N+N94R+D111A+T114I+S216P+L227G+T231R+N233R+P256T、
から選択される置換組を含んで成る請求項１〜２及び５のいずれか１項記載のリパーゼ。
【請求項７】
（ａ）配列番号２のアミノ酸１〜269の配列に対して少なくとも50％の同一性を有し；
（ｂ）リパーゼ活性を有し；そして
（ｃ）配列番号2のアミノ酸１〜269の配列に比較して、次の位置：9, 30, 38, 39, 63, 112, 114, 115, 117, 122, 128, 130, 136, 154, 155, 156, 161 , 163, 168, 185, 190, 239, 及び246から選択された少なくとも１つの位置で置換を含んで成るリパーゼ。
【請求項８】
（ａ）配列番号２のアミノ酸１〜269の配列に対して少なくとも50％の同一性を有し；
（ｂ）リパーゼ活性を有し；そして
（ｃ）配列番号2のアミノ酸１〜269の配列に比較して、次の置換：E1N;Q4H;N8L,Q;Q9H;N11C,D,H,L,P,S; G23E; D27I; P29T; A30T,V; T37K,M; G38A,D,F,H,I,K,L,M,N,P,Q,S,T,W,Y; N39H,S; D57N; G61R; V63R; N71I,S; N73Q,Y; I76T; I86F,L; E87H; G91F,K,L,M,V,Y; N94Q; F95*; D96*; N101Q; D111E; G112A; T114I; S115L; W117C,D,E,F,G,H,I,K,L,P,S,T,V,Y; D122E,N; Q126L; V128A, D130H, H135D, P136H; V141E,L;V154F,I,L;A155V;G156R;G161A,E;N162G,S;G163A,C,D,E,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y; V168M; L185M; G190C,D; R205I; G240L; G246A; N251Q,S;及びL269F,Hから選択された少なくとも１つの置換を含んで成るリパーゼ。
【請求項９】
（ａ）配列番号２のアミノ酸１〜269の配列に対して少なくとも50％の同一性を有し；
（ｂ）リパーゼ活性を有し；そして
（ｃ）配列番号2のアミノ酸１〜269の配列に比較して、次の置換：
D27R+N33Q+G91A+D96E+L97Q+D111A+T231R+N233R+P256T;
N33Q+E210D+T231R+N233R;
N33Q+D111A+T231R+N233R;
N33Q+G91T+T231R+N233R;
N33Q+E219D+T231R+N233R;
N33Q+W117L+T231R+N233R;
D27Q+N33Q+T231R+N233R;
N33Q+G91T+T231R+N233R;
D27S+N33Q+G91A+D96E+L97Q+D111A+S216P+T231R+N233R+P256T;
D27R+N33Q+G91N+N94R+D111A+T231R+N233R+P256T;
D27R+N33Q+G91T+N94S+D111A+S216P+L227G+T231R+N233R+P256T;
Q4R+N33Q+T231R+N233R;
N33Q+T231R+N233R+Q249R;
N33Q+D96W+T231R+N233R;
D27V+N33Q+V60S+D96W+T231R+N233R+Q249R;
D27V+N33Q+V60S+T231R+N233R+Q249R;
Q9H+N33Q+D102E+T231R+N233R;
N33Q+D111E+T231R+N233R;
N33Q+D122E+T231R+N233R;
D27R+N33Q+G91N+N94R+D111A+S216P+L227G+T231R+N233R+P256T;
N33Q+D167E+T231R+N233R;
N33Q+G91N+T231R+N233R;
N33Q+T231R+N233R+P256T;
D27R+N33Q+G91A+L93*+N94*+F95*+D96*+D111A+T231R+N233R+P256T;
N11R+N33Q+T231R+N233R;
N33Q+N39H+T231R+N233R;
N33Q+P229R+T231R+N233R;
D27R+N33Q+G91N+N94R+D111A+G163K+S216P+L227G+T231R+N233R+P256T;
N33Q+G91T+G163K+T231R+N233R;
D27R+N33Q+G91A+D96E+L97Q+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+G91A+D96E+L97Q+D111A+S216P+T231R+N233R+P256T;
N33Q+E87A+T231R+N233R;
N33Q+E56Q+T231R+N233R;
N33Q+E210V+T231R+N233R;
N33Q+E56K+T231R+N233R;
N33Q+T231R+N233R+D254G;
N33Q+D96S+T231R+N233R;
N33Q+D122N+T231R+N233R;
N26A+N33Q+T231R+N233R;
N33Q+N162T+T231R+N233R;
N33Q+A150V+N162G+T231R+N233R;
N33Q+I90L+G163L+T231R+N233R;
N33Q+T231R+N233R+G240L;
D27R+N33Q+G91A+D96E+D111A+T231R+N233R+D254G+P256T;
D27R+N33Q+G91A+N94S+D111A+T231R+N233R+P256T;
N33Q+N200S+T231R+N233R;
N33Q+N39S+T231R+N233R;
N33Q+E210R+T231R+N233R;
N33Q+N39H+T231R+N233R+D254R;
N33Q+T231R+N233R+D254R;
N33Q+N94R+T231R+N233R;
N33Q+D96R+T231R+N233R;
D27N+N33Q+T231R+N233R;
D27N+N33Q+E56R+T231R+N233R;
N33Q+L227F+T231R+N233R;
N33Q+N73Y+G225P+T231R+N233R;
N33Q+G225P+T231 R+N233R;
N33Q+T231R+N233R+D254S;
N33Q+D96G+T231R+N233R;
N33Q+D96N+T231R+N233R+D254S;
N33Q+T231R+N233R+D254G;
N33Q+D130H+T231R+N233R;
N33Q+E87A+T231R+N233R;
N33Q+T231R+N233R+E239D;
N33Q+D111A+T231R+N233R+D254G;
N33Q+E210V+T231R+N233R+D254S;
N11R+N33Q+E210V+T231R+N233R+D254S;
N33Q+G91T+G163K+T231R+N233R+D254G;
N33Q+G91T+G163K+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+T231R+N233R+D254S;
Q4R+D27R+N33Q+G91T+N94S+D111A+S216P+L227G+T231R+N233R+P256T;
N33Q+G91T+N94S+D111A+V176I+T231R+N233R;
Q4R+D27R+N33Q+G91T+N94S+D111A+E210D+S216P+L227G+T231R+N233R+P256T;
Q4R+D27Q+N33Q+G91T+N94S+D111A+S216P+L227G+T231R+N233R+P256T;
N33Q+G91T+N94S+D111A+T231R+N233R+P256T;
N33Q+G177A+T231R+N233R;
N33Q+T231R+N233R+G246A;
D27N+N33Q+G91T+G163K+T231R+N233R+D254S;
D27Q+N33Q+G91T+G163K+E219D+T231R+N233R;
N33Q+G91T+E219D+T231R+N233R;
K98I+T231R+N233R+N251S;
N33Q+G163R+T231R+N233R;
N33Q+G163N+T231R+N233R;
N33Q+G163C+T231R+N233R;
N33Q+G163Q+T231R+N233R;
N33Q+G163E+T231R+N233R;
N33Q+G163H+T231R+N233R;
N33Q+G163I+T231R+N233R;
N33Q+G163P+T231R+N233R;
N33Q+G163D+T231R+N233R;
N33Q+G91K+T231R+N233R;
N33Q+G91M+T231R+N233R;
N33Q+G91F+T231R+N233R;
N33Q+G91S+T231R+N233R;
N33Q+G91W+T231R+N233R;
N33Q+G91Y+T231R+N233R;
N33Q+G163T+T231R+N233R;
N33Q+G163W+T231R+N233R;
N33Q+G163Y+T231R+N233R;
N33Q+G163V+T231R+N233R;
N33Q+G91C+T231R+N233R;
N33Q+G91Y+Q126L+T231R+N233R;
N33Q+G91M+G161E+T231R+N233R;
N33Q+V128A+T231R+N233R;
N33Q+G163V+L185M+T231R+N233R;
N33Q+G38A+T231R+N233R;
N33Q+G163A+T231R+N233R;
N33Q+G91T+N94S+D111A+T231R+N233R;
N33Q+G38A+G163A+T231R+N233R;
N33Q+G163M+T231R+N233R;
N33Q+G91V+T231R+N233R;
N33Q+D111A+T231R+N233R+Q249R;
N33Q+D111A+T231R+N233R+D254S;
D27R+N33Q+G91A+D96E+L97Q+D111A+T231R+N233R+D254S+P256T;
D27R+N33Q+G91A+D96E+L97Q+D111A+T231R+N233R+D254G+P256T;
N33Q+G91T+N94R+T231R+N233R+D254S;
N33Q+G91T+N94R+D111A+W117L+T231R+N233R;
N33Q+W117L+T231R+N233R+D254S;
N33Q+T231R+N233R+P256T;
N33Q+T231R+N233R+D242E;
N33Q+E87R+T231R+N233R;
N33Q+E56R+T231R+N233R;
N33Q+N162G+T231R+N233R;
N33Q+G91L+T231R+N233R;
N33Q+E87H+T231R+N233R;
N33Q+D96N+T231R+N233R+Q249R;
N33Q+G91T+N94R+T231R+N233R+D254S;
N33Q+L227F+T231R+N233R+D254S;
D27R+N33Q+G91T+D96E+L97Q+D111A+T231R+N233R+D254S+P256T;
N33Q+G163A+T231R+N233R;
D27R+N33Q+G91T+D96E+D111A+T231R+N233R+D254S+P256T;
N33Q+G91T+N94R+T231R+N233R;
N33Q+T231R+N233R+D254A;
N33Q+T231R+N233R+D254N;
N33Q+T231R+N233R+D254Q;
N33Q+T231R+N233R+D254I;
N33Q+T231R+N233R+D254L;
N33Q+T231R+N233R+D254K;
N33Q+T231R+N233R+D254M;
N33Q+S216P+L227G+T231R+N233R+Q249R;
D27V+N33Q+V60S+G91T+D96W+T231R+N233R+Q249R;
N33Q+D96N+L227G+T231R+N233R+Q249R;
D27R+N33Q+L227G+T231R+N233R;
D27R+N33Q+L227G+T231R+N233R+Q249R;
N33Q+E219D+L227G+T231R+N233R+Q249R;
D27Q+N33Q+L227G+T231R+N233R+Q249R;
N33Q+W117L+L227G+T231R+N233R+Q249R;
D5E+N33Q+W117L+L227G+T231R+N233R+Q249R;
D27Q+N33Q+E219D+L227G+T231R+N233R+Q249R;
N33Q+D96E+E219D+L227G+T231R+N233R+Q249R;
D27R+N33Q+E56K+G91N+N94R+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+E56Q+D57N+G91N+N94R+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+S216P+L227G+T231R+N233R+D254S+P256T;
D27R+N33Q+E56S+G91N+N94R+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+G91N+N94R+D111A+S216P+L227G+T231R+N233R+D254S+P256T;
D27R+N33Q+G91N+N94R+D111A+S216P+L227G+T231R+N233R+D254S+P256T;
D27R+N33Q+G91N+N94R+D111S+A155V+S216P+L227G+T231R+N233R+D254S+P256T;
D27R+N33Q+G91N+N94R+D111S+S216P+L227G+T231R+N233R+D254S+P256T;
N33Q+D111A+T231R+N233R+D254S;
N33Q+D111A+W117L+T231R+N233R+D254S;
N33Q+T231R+N233R+P256A;
N33Q+T231R+N233R+P256N;
N33Q+T231R+N233R+P256G;
N33Q+T231R+N233R+P256H;
N33Q+T231R+N233R+P256L;
N33Q+T231R+N233R+P256M;
N33Q+T231R+N233R+P256S;
N33Q+T231R+N233R+P256W;
N33Q+T231R+N233R+P256Y;
N33Q+T231R+N233R+P256F;
N33Q+T231R+N233R+P256V;
N33Q+G91M+G163W+T231R+N233R;
N33Q+G91M+G163T+T231R+N233R;
N33Q+G91M+G163D+T231R+N233R;
N33Q+G91K+G163W+T231R+N233R;
N33Q+G91T+G163W+T231R+N233R;
N33Q+V176N+T231R+N233R;
N33Q+V176D+T231R+N233R;
N33Q+W117F+T231R+N233R;
N33Q+G91T+N94S+D111A+V176I+T231R+N233R+D254S;
N33Q+V176I+T231R+N233R;
N33Q+D111N+T231R+N233R;
N33Q+D111N+G225P+T231 R+N233R;
N33Q+D111N+S216P+T231R+N233R;
D27R+N33Q+G91T+N94R+D111A+S216P+L227G+T231R+N233R;
N33Q+G91M+G163P+T231R+N233R;
N33Q+G91T+G163A+T231R+N233R;
N33Q+W117D+T231R+N233R;
N33Q+W117H+T231R+N233R;
N33Q+W117C+T231R+N233R;
N33Q+W117K+T231R+N233R;
N33Q+W117V+T231R+N233R;
N11S+N33Q+T231R+N233R;
N33Q+W117E+V176K+T231R+N233R;
N33Q+W117G+T231R+N233R;
N33Q+W117P+T231R+N233R;
N33Q+W117S+T231R+N233R;
N33Q+W117T+T231R+N233R;
N33Q+W117I+T231R+N233R;
D27R+N33Q+L227G+T231R+N233R+Q249R+D254S;
N33Q+S115L+T231R+N233R;
N33Q+G38A+G91T+G163K+T231R+N233R+D254S;
N33Q+V176M+T231R+N233R;
N33Q+V176H+T231R+N233R;
N33Q+V176A+T231R+N233R;
D27V+N33Q+L227F+T231R+N233R+Q249R;
N33Q+W117Y+T231R+N233R;
N33Q+W117Y+V176D+T231R+N233R;
D27V+N33Q+G91A+N94R+D111A+G163K+L227F+T231R+N233R+Q249R;
D27V+N33Q+G91A+N94R+D111A+G163K+L227F+T231R+N233R+Q249R+D254S;
D27R+N33Q+P136H+L227G+T231R+N233R+Q249R+D254S;
N11R+N33Q+T231R+N233R+T244S;
N33Q+G91T+D96N+D111A+V176I+T231R+N233R+D254S;
N33Q+G91T+N94S+D111A+V176I+T231R+N233R+D254S;
N33Q+G161A+T231R+N233R;
N33Q+G38I+G177A+T231R+N233R;
N33Q+N101Q+T231R+N233R;
N33Q+N94Q+T231R+N233R;
N33Q+G161A+T231R+N233R;
N11Q+N33Q+T231R+N233R;
N8Q+N33Q+T231R+N233R;
N33Q+T231R+N233R+N251Q;
N33Q+N200Q+T231R+N233R;
N33Q+G177A+T231R+N233R;
N33Q+N73Q+T231R+N233R;
N33Q+I86L+T231R+N233R;
N33Q+K98I+G163K+T231R+N233R;
D27R+N33Q+G91T+D96E+D111A+G163K+T231R+N233R+D254S+P256T;
D27R+N33Q+G91T+D96E+D111A+G163A+T231R+N233R+D254S+P256T;
D27R+N33Q+S216P+L227G+T231R+N233R+Q249R;
N33Q+K98I+G163K+N200Q+T231R+N233R+N251S;
N33Q+G38S+G163K+T231R+N233R;
D27R+N33Q+G38A+G91T+D96E+D111A+T231R+N233R+D254S+P256T;
N33Q+G38Y+T231R+N233R;
D27R+N33Q+G91T+N94R+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+G91T+N94R+D111A+S216P+L227G+T231R+N233R+P256T;
N33Q+G38N+N73Q+T231R+N233R;
N33Q+G38D+R84E+T231R+N233R;
N33Q+G38Q+T231R+N233R;
N33Q+G38I+T231R+N233R;
N33Q+G38K+T231R+N233R;
N33Q+G38F+T231R+N233R;
N33Q+G38H+N200Q+T231R+N233R+N251S;
N33Q+G38L+T231R+N233R;
N33Q+G38M+T231R+N233R;
N33Q+G38F+T231R+N233R;
N33Q+G38P+T231R+N233R;
N33Q+G38T+T231R+N233R;
N11R+N33Q+G91T+W117I+G163K+T231R+N233R+D254S;
D27R+N33Q+G38A+G91T+D96E+D111A+G163K+T231R+N233R+D254S+P256T;
N11R+N33Q+G91T+W117I+G163K+T231R+N233R+D254S;
D27R+N33Q+G38A+G91T+D96E+D111A+G163A+T231R+N233R+D254S+P256T;
D27R+N33Q+V176Q+L227G+T231R+N233R+Q249R+D254S;
N33Q+W117I+V176Q+T231R+N233R+P256A;
N33Q+G38A+G163A+T231R+N233R+P256A;
N33Q+W117I+V176Q+T231R+N233R;
N33Q+G177A+T231R+N233R+G246A;
E1N+N33Q+T231R+N233R;
N33Q+G38H+T231R+N233R;
N33Q+G91A+N94K+D111A+G163K+L227F+T231R+N233R+Q249R+D254S;
N11R+N33Q+G91T+G163K+V176Q+T231R+N233R+D254S;
N33Q+K98I+T231R+N233R;
D27R+N33Q+W117I+V176Q+L227G+T231R+N233R+Q249R+D254S;
N11R+N33Q+G38A+G91T+G163K+T231R+N233R+D254S;
N33Q+G163W+T231R+N233R;
N33Q+G38A+G163A+T231R+N233R;
D27R+N33Q+G91T+D96E+L97Q+D111A+T231R+N233R+D254S+P256T;
N33Q+T231R+N233R+D254Q;
N11R+N33Q+G91T+S115L+G163K+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+V176W+T231R+N233R+D254S;
N33Q+G163D+T231R+N233R;
N33Q+G163P+T231R+N233R;
E1D+N33Q+G91T+N94R+D111A+W117L+T231R+N233R+D254S;
N33Q+G91T+N94R+D111A+W117L+V176W+T231R+N233R;
Q4P+D27R+N33Q+G91N+N94R+D111A+L206F+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+T37K+N711+G91N+N94R+K98I+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+E43K+K46M+I90V+G91N+N94R+D111A+T114I+S216P+L227G+T231R+N233R+P256T;
N33Q+W117S+T231R+N233R;
N33Q+G61R+V63R+G156R+V176W+T231R+N233R+P256I;
N33Q+D96N+G156R+V176W+T231R+N233R;
N33Q+G156R+V176W+T231R+N233R+Q249R;
N33Q+G91T+N94S+D111A+G163T+V176W+T231R+N233R;
N33Q+G91T+N94S+D111A+S115L+G163T+V176I+T231R+N233R;
N11R+D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+G163T+S216P+L227G+T231R+N233R+D254S+P256T;
D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+G163T+S216P+L227G+T231R+N233R+D254S+P256T;
N11R+D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+S216P+L227G+T231R+N233R+D254S+P256T;
D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+S216P+L227G+T231R+N233R+D242E+D254S+P256T;
D27R+N33Q+G38A+E56Q+D57N+G91N+N94R+D111S+S216P+L227G+T231R+N233R+D254S+P256T;
Q4R+D27Q+N33Q+G91T+N94S+E99D+D111A+E210D+S216P+L227G+T231R+N233R+P256L;
N33Q+G38A+G91T+G163A+T231R+N233R+D254S;
N33Q+G38A+G163A+T231R+N233R+D254I;
N11R+N33Q+I90L+G163L+T231R+N233R;
N11R+N33Q+I90L+G163L+T231R+N233R+D254S;
N11R+N33Q+E56Q+G91T+G163K+V176Q+T231R+N233R+D254S;
N11R+D27R+N33Q+G91T+D96E+D111A+G163K+T231R+N233R+D254S+P256T;
N11R+N33Q+G38A+G91T+G112A+G163A+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+E210D+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+T231R+N233R+D254I;
N11R+N33Q+G91T+G163K+V176T+T231R+N233R+D254S;
N11R+N33Q+G91T+G163P+T231R+N233R+D254S;
N11R+N33Q+G91M+G163T+T231R+N233R+D254S;
N11R+N33Q+G38A+G91T+G163K+V176D+T231R+N233R+D254S;
N33Q+E56Q+G156R+V176W+T231R+N233R;
E1D+N33Q+G38A+G91T+N94R+D111A+W117L+V176W+T231R+N233R;
N33Q+G163K+G177A+T231R+N233R+G246A;
N11R+N33Q+E56Q+G91T+G163K+T231R+N233R+D254S;
N11R+N33Q+I90L+G163K+T231R+N233R+D254S;
D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+S216P+L227G+T231R+N233R+Q249R+D254S+P256T;
D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+S216P+E219D+L227G+T231R+N233R+D254S+P256T;
N11R+N33Q+I90L+G91T+N94S+D96E+G163K+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+V176I+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+V176Q+T231R+N233R+D254S;
N11R+N33Q+G91T+G163A+V176T+T231R+N233R+D254S;
N11R+N33Q+G91T+G163L+V176I+T231R+N233R+D254S;
N11R+N33Q+G91T+G163L+V176T+T231R+N233R+D254S;
N11R+N33Q+G91T+G163L+T231R+N233R+D254S;
N11R+N33Q+G91T+G163P+T231R+N233R+D254S;
N11R+N33Q+G91T+G163P+V176I+T231R+N233R+D254S;
N11R+N33Q+G91T+G163L+T231R+N233R+D254S+P256N;
D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+G163T+S216P+L227G+T231R+N233R+Q249R+D254S+P256T;
Q4R+D27Q+N33Q+G91T+N94S+E99D+D111A+G163A+E210V+S216P+L227G+T231R+N233R+P256L;
Q4R+D27Q+N33Q+G91T+N94S+E99D+D111A+V176I+E210V+S216P+L227G+T231R+N233R+P256L;
N33Q+E210Y+T231R+N233R+D254Y+I255F;
N33Q+L93F+D102Y+T231R+N233R;
D27R+N33Q+L227G+T231R+N233R+Q249R+D254S;
N11S+N33Q+T231R+N233R;
N11R+N33Q+T231R+N233R;
N33Q+G38A+G91T+G163K+T231R+N233R+D254S;
N33Q+W117Y+V176T+T231R+N233R;
N8L+N11R+N33Q+G91T+G163K+T231R+N233R+D254S;
E1N+N33Q+G38A+G91T+G163P+V176F+T231R+N233R;
N11R+N33Q+G38A+G91T+G163P+V176G+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+T231R+N233R+D254A+P256F;
N11R+N33Q+G91T+G163K+T231R+N233R+P256F;
N11R+N33Q+G91T+G163K+T231R+N233R+D254S+P256F;
N11R+N33Q+G38A+G91T+G156R+G163K+V176T+T231 R+N233R+D254S;
N33Q+G91K+D96S+G163T+T231R+N233R+Q249R;
N11R+N33Q+G91T+G163N+T231R+N233R+D254S;
N11R+N33Q+G91T+G163T+T231R+N233R+D254S;
N11R+N33Q+G91T+G163W+T231R+N233R+D254S;
N11R+N33Q+G91K+G163K+T231R+N233R+D254S;
N11R+G23E+N33Q+G91T+G163K+T231R+N233R+D254S;
N11R+N33Q+G91T+V141 E+G163K+T231R+N233R+D254S;
N11R+N33Q+L52R+G91T+G163K+T231R+N233R+D254S;
N11R+N33Q+G91T+V141L+G163K+T231R+N233R+D254S;
N11R+N33Q+T37K+G91T+G163K+T231R+N233R+D254S;
N11R+N33Q+A68V+G91T+G163K+T231 R+N233R+D254S;
N11R+N33Q+G91T+G163A+V176I+T231R+N233R+D254S;
N11R+N33Q+T37M+G91T+G163P+V176T+T231R+N233R+D254S;
N11R+N33Q+G91T+G163L+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+T231R+N233R+D254S+P256I;
N33Q+G38S+G156R+G163K+V176W+T231R+N233R;
N11R+D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+G163K+S216P+L227G+T231R+N233R+D254S+P256T;
N11R+N33Q+G38A+G91T+G163P+V176G+T231R+N233R+D254S;
N11R+N33Q+G38A+G91T+G163Q+V176G+T231R+N233R+D254S;
N11R+N33Q+G38A+G91T+G163T+V176G+T231R+N233R+D254S;
N11R+N33Q+G38A+G91T+N94R+G163P+V176G+T231R+N233R+D254S;
E1*+N11R+N33Q+G38A+G91N+N94R+G 163P+ V176G+T231R+N233R+D254S;
E1N+N11R+N33Q+G38A+G91T+G163P+V176F+T231R+N233R;
E1N+F10L+N11R+N33Q+G38A+G91T+G163P+V176F+T231 R+N233R;
E1N+N33Q+G38A+G91T+G163P+V176F+T231R+N233R+D254S;
E1N+N33Q+G38A+G91T+D111A+G163P+V176F+T231 R+N233R;
E1N+N33Q+G38A+G91T+G163P+V176F+L227F+T231R+N233R;
E1N+N11R+N33Q+G38A+G91T+D111A+G163P+V176F+T231R+N233R;
E1N+N33Q+G38A+G91T+G163P+V176F+L227F+T231R+N233R+D254S;
E1N+N33Q+G38A+G91T+G163P+V176F+T231R+N233R+D254S+I255A+P256Q;
E1N+N11R+N33Q+G38A+G91T+D111A+G163P+V176F+T231R+N233R+D254S;
N33Q+G156R+V176W+T231R+N233R+P256I;
N33Q+G91T+N94S+D111A+G156R+G163T+V176W+T231R+N233R;
N33Q+G91T+N94S+D111A+G156R+G163T+V176I+T231R+N233R;
N11R+N33Q+G38A+G91T+D102G+S115L+G163K+T231R+N233R+D254S+P256T;
N11R+N33Q+G38A+G91T+S115L+G163K+T231R+N233R+D254S+P256T;
E1N+N11R+N33Q+G91T+G163A+T231R+N233R+G246A+D254S;
N11R+D27R+N33Q+D57G+G91T+D96E+D111A+G163K+T231R+N233R+D254S+P256T;
N33Q+D96N+G156R+V176W+T231R+N233R+Q249R;
N33Q+I86F+L93F+D102Y+E210Y+L227F+T231R+N233R+D254Y+I255F+L269F;
N33Q+I86F+L93F+D102Y+E210Y+L227F+T231R+N233R+D254Y+I255F;
N11C+N33Q+G91T+G163K+T231R+N233R+D254S;
N11L+N33Q+G91T+G163K+T231R+N233R+D254S;
N11H+N33Q+G91T+G163K+T231R+N233R+D254S;
N11D+N33Q+G91T+G163K+T231R+N233R+D254S;
N11R+N33Q+G91T+D96W+G163K+T231R+N233R+D254S;
D27R+N33Q+G91T+D96E+L97Q+D111A+G163K+T231R+N233R+D254S+P256T;
N11P+N33Q+G91T+G163K+T231R+N233R+D254S;
Q4R+D27N+N33Q+G38A+G91T+N94S+E99D+D111A+V176I+E210V+S216P+L227G+T231R+N233R+P256L;
N11R+N33Q+E56Q+G163K+T231R+N233R+D254S;
N11R+N33Q+G91T+G163A+T231R+N233R+D254S;
N11R+N33Q+G91T+G163P+T231R+N233R+D254S;
N11R+N33Q+G91T+G163K+L227G+P229R+T231R+N233R+D254S;
N33Q+E87K+T231R+N233R;
N33Q+N94K+T231R+N233R;
N33Q+D96Y+T231R+N233R;
N33Q+K98I+T231R+N233R;
A30V+N33Q+K98I+T231R+N233R;
N33Q+E87K+D96E+T231R+N233R;
N26I+N33Q+T231R+N233R;
A30T+N33Q+T231R+N233R;
N33Q+G91V+T231R+N233R;
N33Q+G91A+T231R+N233R;
N33Q+G91V+L97M+T231R+N233R;
N33Q+K98I+T231R+N233R;
N33Q+L69I+G91E+T231R+N233R;
P29T+N33Q+T231R+N233R;
N33Q+G91V+T231R+N233R;
N33Q+K98I+T231R+N233R;
N33Q+G91E+T231R+N233R;
N33Q+N94K+T231R+N233R;
D27R+N33Q+G91N+N94R+K98I+D111A+N162S+S216P+L227G+T231R+ N233R+P256T;
D27R+N33Q+T37K+N711+G91N+N94R+K98I+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+N39S+G91N+N94R+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+I76T+G91N+N94R+R108M+D111A+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+L52I+V60E+G91N+N94R+D111A+T114I+V168M+E210D+S216P+L227G+T231R+N233R+P256T;
Q4P+D27R+N33Q+G91N+N94R+D111A+R205I+L206F+S216P+L227G+T231R+N233R+P256T;
Q4H+D27R+N33Q+G91N+N94R+D111A+V154L+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+G91N+N94R+D111A+V154I+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+N71S+G91N+N94R+D111A+H135D+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+G91N+N94R+K98I+D111A+S216P+L227G+T231 R+N233R+P256T;
D27R+N33Q+G91N+N94R+L97M+D111A+S216P+T226N+L227G+T231R+N233R+P256T+L269H;
D27R+N33Q+G91N+N94R+D111A+T114I+R179T+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+G91N+N94R+D111A+S216P+L227G+T231R+N233R；
G23E+D27R+N33Q+L52R+G91N+N94R+D111A+T114I+V141E+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+E43K+K46M+I90V+G91N+N94R+D111A+T114I+S216P+L227G+T231R+N233R+P256T;
D27R+A30V+N33Q+G91N+N94R+G109A+D111A+G190D+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+A49T+G91N+N94R+D111A+Y138F+G163R+S216P+L227G+T231R+N233R+P256T;
N26H+D27R+N33Q+G91N+N94R+D111A+V154F+G190C+S216P+L227G+T231R+N233R+P256T;
N33Q+G91T+D96E+K98T+T114I+G163S+E210V+T231R+N233R+D254K+P256A;
N33Q+G91T+D96E+K98T+T114I+T231R+N233R+G163S;
N33Q+G91T+D96E+K98T+T114I+G163K+E210D+T231R+N233R;
N33Q+G91T+T114I+G163K+E210D+T231R+N233R+D254G+P256A;
D27R+N33Q+G91T+T114I+G163W+E210D+T231R+N233R;
D27N+N33Q+G91T+T114I+G163S+E210D+T231R+N233R+P256T;
N33Q+G91T+T114I+G163K+E210D+T231R+N233R;
N33Q+G38W+G91T+T114I+G163K+E210V+T231R+N233R;
N33Q+G38W+G91T+T114I+G163K+E210D+T231R+N233R+P256T;
D27I+N33Q+G91T+D96E+K98T+T114I+G163K+E210D+T231R+N233R+P256T;
N33Q+G91T+T114I+E210V+T231R+N233R+D254K+P256A;
N33Q+G91A+N94K+D111A+G163K+L227F+T231R+N233R+Q249R;
G23E+D27R+N33Q+L52R+G91N+N94R+D111A+T114I+V141E+S216P+L227G+T231R+N233R+P256T;
D27R+N33Q+E43K+K46M+I90V+G91N+N94R+D111A+T114I+S216P+L227G+T231R+N233R+P256T;
N33Q+G91T+K98I+T114I+G163K+T231R+N233R+D254S;
N33Q+G91T+K98I+G163K+T231R+N233R+D254S+P256L;
N33Q+G91T+T114I+G163K+T231R+N233R+D254S+P256L;
G23E+D27R+N33Q+L52R+G91N+N94R+D111A+T114I+V141E+S216P+L227G+T231R+N233R+P256T; 及び
D27R+N33Q+E43K+K46M+I90V+G91N+N94R+D111A+T114I+S216P+L227G+T231R+N233R+P256T、から選択された置換組を含んで成るリパーゼ。
【請求項１０】
薬剤として使用のためにプロテアーゼ又はアミラーゼと組合しての請求項１〜２及び５〜６のいずれか１項記載のリパーゼ。
【請求項１１】
薬剤として使用のためにプロテアーゼ及びアミラーゼと組合しての請求項１〜２及び５〜６のいずれか１項記載のリパーゼ。
【請求項１２】
（i）前記プロテアーゼが、
ａ）配列番号３のアミノ酸１〜274の配列を有するプロテアーゼ、
ｂ）配列番号４のアミノ酸１〜188の配列を有するプロテアーゼ、及び
ｃ）配列番号５のアミノ酸１〜188の配列を有するプロテアーゼから成る群から選択されたプロテアーゼに対して少なくとも70％の同一性を有し；
（ii）前記アミラーゼが、
ａ）配列番号６のアミノ酸１〜481の配列を有するアミラーゼ、
ｂ）配列番号７のアミノ酸１〜481の配列を有するアミラーゼ、及び
ｃ）配列番号８のアミノ酸１〜483の配列を有するアミラーゼから成る群から選択されたアミラーゼに対して少なくとも70％の同一性を有する請求項10又は11記載のプロテアーゼ及び/又はアミラーゼと組合してのリパーゼ。
【請求項１３】
消化障害、膵臓外分泌機能不全、膵炎、嚢胞性線維症、糖尿病Ｉ型及び/又は糖尿病II型の処理のための薬剤の製造のためへの請求項１〜２及び５〜６のいずれか１項記載のリパーゼ又はリパーゼ混合物の使用。
【請求項１４】
プロテアーゼ又はアミラーゼの使用をさらに含んで成る請求項13記載の使用。
【請求項１５】
プロテアーゼ及びアミラーゼの使用をさらに含んで成る請求項13記載の使用。
【請求項１６】
前記プロテアーゼ及び/又はアミラーゼが、請求項12に記載の通りである請求項14又は15記載の使用。
【請求項１７】
消化障害、膵臓外分泌機能不全、膵炎、嚢胞性線維症、糖尿病Ｉ型及び/又は糖尿病II型の処理への使用のためへの請求項１〜２及び５〜６のいずれか１項記載のリパーゼ。
【請求項１８】
プロテアーゼ又はアミラーゼと組合しての請求項17記載のリパーゼ。
【請求項１９】
プロテアーゼ及びアミラーゼと組合して請求項17記載のリパーゼ。
【請求項２０】
前記プロテアーゼ及び/又はアミラーゼが請求項12記載の通りである請求項18又は19記載のリパーゼ。
【請求項２１】
請求項１〜２及び５〜６のいずれか１項記載のリパーゼ又はリパーゼ混合物、及び少なくとも１つの医薬的に許容できる助剤材料を含んで成る医薬組成物。
【請求項２２】
プロテアーゼ又はアミラーゼをさらに含んで成る請求項21記載の組成物。
【請求項２３】
プロテアーゼ及びアミラーゼをさらに含んで成る請求項21記載の組成物。
【請求項２４】
前記プロテアーゼ及び/又はアミラーゼが請求項12記載の通りである請求項22又は23記載の組成物。
【請求項２５】
治療的有効量の請求項１〜２及び５〜６のいずれか１項記載のリパーゼ又はリパーゼ混合物を投与することによる、消化障害、膵臓外分泌機能不全、膵炎、嚢胞性線維症、糖尿病Ｉ型及び/又は糖尿病II型の処理方法。
【請求項２６】
治療有効量のプロテアーゼ又はアミラーゼを投与することをさらに含んで成る請求項25記載の方法。
【請求項２７】
治療有効量のプロテアーゼ及びアミラーゼを投与することをさらに含んで成る請求項25記載の方法。
【請求項２８】
前記プロテアーゼ及び/又はアミラーゼが請求項12記載の通りである請求項26又は27記載の方法。
【請求項２９】
（ａ）配列番号２のアミノ酸１〜269の配列に対して少なくとも50％の同一性を有し；そして
（ｂ）リパーゼ活性を有し；そして
（ｃ）次の置換：N26I, D27Q, D27R, D27Y, P29T, A30T, A30V, T32I, N33Q, N33T, N33Y, P42L, E43D, E43K, E43M, E43V, A49T, E56A, E56C, E56K, E56R, E56S, D57A, D57G, D57N, V60L, L69I, E87K,G91A, G91E, G91N, G91R, G91S, G91T, G91V, G91W, L93F, N94K, N94R, N94S, D96E, D96G, D96L, D96N, D96S, D96V, D96W, D96Y, L97M,L97Q, K98I, E99D, E99K, E99P, E99S, E99T, D111A, D111S, T114I, L147S, G163K, E210D, S216P, L227G, T231 R, N233R, D234K, E239V, Q249R, N251S, D254N, P256T, G263Q, L264A, I265T, G266D, T267A及びL269Nから選択された少なくとも１つの置換を含んで成り、ここで個々の位置は配列番号２のアミノ酸１〜269の位置に対応し；そして
（ｄ）但し、
（i）配列番号１のアミノ酸１〜269を有するリパーゼではなく、そして
（ii）(i)のリパーゼの変異体N33Qではない、親リパーゼの変異体である、薬剤として使用するためのリパーゼ。
【請求項３０】
（ａ）配列番号２のアミノ酸１〜269に対して少なくとも50％の同一性を有し；そして
（ｂ）リパーゼ活性を有し；そして
（ｃ）位置30、42、114及び/又は163の少なくとも１つの位置で少なくとも１つの置換を含んで成る（ここで、個々の位置は配列番号２のアミノ酸１〜269の位置に対応する）、親リパーゼの変異体である、薬剤として使用するためのリパーゼ。
【請求項３１】
（ａ）配列番号２のアミノ酸１〜269に対して少なくとも50％の同一性を有し；そして
（ｂ）リパーゼ活性を有し；そして
（ｃ）次の置換：A30T、A30V、P42L、T114I及びG163Kから選択された少なくとも１つの置換を含んで成る（ここで、個々の位置は配列番号２のアミノ酸１〜269の位置に対応する）、親リパーゼの変異体である、薬剤として使用するためのリパーゼ。
【請求項３２】
（ａ）配列番号２のアミノ酸１〜269に対して少なくとも50％の同一性を有し；そして
（ｂ）リパーゼ活性を有し；そして
（ｃ）次の変異体：
LV1232 G91A+D96W+E99K+G263Q+L264A+I265T+G266D+T267A+L269N;
LV1330 N33Q+D96S+T231R+N233R+Q249R;
LV1855 D27R+G91A+D111A+S216P+L227G+P256T;
LV1857 D27R+G91N+N94R+D111A+S216P+L227G+P256T;
LV1865 D27R+G91T+N94S+D111A+S216P+L227G+P256T;
LV1874 D27R+G91S+D111A+S216P+L227G+P256T;
LV1889 D27R+G91T+D96N+D111A+S216P+L227G+P256T;
LVA049 N33Q+G163K+T231R+N233R;
LVAR0002b T32I+G91V+T231R+N233R;
LVAR0003 K98I+T231R+N233R;
LVAROO11a G91A+T231R+N233R;
LVAR0013 G91V+T231R+N233R;
LVAR0014 N33Y+G91W+N94K+T231R+N233R;
LVAR0015 P42L+D57N+G91E+T231R+N233R;
LVAR0016 K98I+T231R+N233R;
LVAR0017 V60L+G91V+T231R+N233R;
LVAR0032 D57G+L93F+T231R+N233R;
LVAR0045 A49T+E56R+E87K+E99S+T231R+N233R;
LVAR0046 E99T+T114I+D254N+T231R+N233R;
LVAR0047 D27Y+E87K+D96L+E99P+T231R+N233R;
LVAR0048 E43K+E56S+E87K+T231R+N233R;
LVAR0050 E56S+E87K+D96L+E99D+T231R+N233R;
LVAR0051 E56A+D57A+T114I+T231R+N233R;
LVAR0052 G91E+T231R+N233R;
LVAR0053 E56K+D96G+D111A+T231R+N233R;
LVAR0054 E87K+D111S+T231R+N233R;
LVAR0055 E43V+G91R+T231R+N233R;
LVAR0056 E56S+E87K+T231R+N233R;
LVAR0057 E87K+G91E+T231R+N233R;
LVAR0058 D27Y+E87K+T231R+N233R;
LVAR0059 E43M+E87K+D96L+E99P+T231R+N233R;
LVAR0061 E56K+E87K+D111A+T231R+N233R;
LVAR0062 E87K+E99P+T231R+N233R;
LVAR0063 E87K+D96L+E99P+T231R+N233R;
LVAR0064 E56C+E87K+T231R+N233R;
LVAR0065 E56R+E87K+D96L+T231R+N233R;
LVAR0066 E43D+E56A+D57A+E87K+D111A+T231R+N233R;
LVAR0067 E56K+E87K+D96L+E99P+T231R+N233R;
LVAR0068 E87K+L147S+T231R+N233R;
LVAR0069 D27Y+E87K+D96L+E99P+T231R+N233R;
LVAR0070 E43D+E87K+D96L+E99P+E239V+T231R+N233R;
LVAR0071 E43K+E56A+E87K+D234K+T231R+N233R;
LVAR0072 D96V+D111A+T231R+N233R; 及び
LVAR0078 N33T+E43V+E56K+D96G+T231R+N233R；
から選択される、親リパーゼの変異体である、薬剤として使用するためのリパーゼ。
【公表番号】特表２０１０−５１２７９５（Ｐ２０１０−５１２７９５Ａ）
【公表日】平成２２年４月３０日（２０１０．４．３０）
【国際特許分類】
【出願番号】特願２００９−５４３０７８（Ｐ２００９−５４３０７８）
【出願日】平成１９年１２月１２日（２００７．１２．１２）
【国際出願番号】ＰＣＴ／ＵＳ２００７／０８７１６８
【国際公開番号】ＷＯ２００８／０７９６８５
【国際公開日】平成２０年７月３日（２００８．７．３）
【出願人】（５００５８６２９９）ノボザイムス アクティーゼルスカブ (164)
【出願人】（５００１７５６０２）ノボザイムス，インコーポレイティド (26)
【出願人】（５０７４１６９９７）ゾルファイ ファーマシューティカルズ ゲゼルシャフト ミット ベシュレンクテル ハフツング (5)
【Ｆターム（参考）】
【公表日】平成２２年４月３０日（２０１０．４．３０）
【国際特許分類】
【出願日】平成１９年１２月１２日（２００７．１２．１２）
【国際出願番号】ＰＣＴ／ＵＳ２００７／０８７１６８
【国際公開番号】ＷＯ２００８／０７９６８５
【国際公開日】平成２０年７月３日（２００８．７．３）
【出願人】（５００５８６２９９）ノボザイムス アクティーゼルスカブ (164)
【出願人】（５００１７５６０２）ノボザイムス，インコーポレイティド (26)
【出願人】（５０７４１６９９７）ゾルファイ ファーマシューティカルズ ゲゼルシャフト ミット ベシュレンクテル ハフツング (5)
【Ｆターム（参考）】
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